antibody

Rabbit anti CD8 Polyclonal

MBS551004

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit anti CD8 Polyclonal

CD8

Rabbit anti Human CD8 Polyclonal purified; cd8; cd-8; polyclonal; PAb

T-cell surface glycoprotein CD8 alpha chain isoform 1; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

CD8

CD8A; MAL

CD8A_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

235

Serum IgG fraction was purified by Protein G affinity chromatography. Purity: >95%

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. This antbody can be stored in working aliquots at 2-8 degree C for one month without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE rat small intestines tissue showing localization of CD8. It was used as primary antibody, dilution 1:200.

Source Note: Polyclonal anti CD8 was produced from sera of rabbits immunized with synthetic protein containing CD8 C terminal antigenic determinants. Presentation: 0.2um filtered 1mg/ml solution in TBS pH 7.4, 50% glycerol, 1% BSA, 0.03% proclin.

Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.

Polyclonal

The cell surface glycoprotein CD8 plays an important role in the development and function of MHC3 class I-restricted T cells. CD8 fulfils its function through interacting via its extracellular domain with MHC class I proteins and via its intracellular domain with the tyrosine kinase Lck (1, 2). An interaction of both the T cell receptor (TCR) and CD8 with MHC proteins enhances the adhesion between the T cell and the antigen- presenting cell.The coreceptor CD8 is expressed as a CD8ab heterodimer on major histocompatibility complex class I-restricted TCRab T cells, and as a CD8aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, natural killer cells, and dendritic cells.

extralymphoid CD8 + t cells resident in tissue from simian immunodeficiency virus sivmac239nef-vaccinated macaques suppress sivmac239 replication ex vivo . J. Virol., Apr 2010; 84: 3362 - 3372. dendritic cell migration limits the duration of CD8 + t-cell priming to peripheral viral antigen . J. Virol., Apr 2010; 84: 3586 - 3594. recombinant yellow fever vaccine virus 17d expressing simian immunodeficiency virus sivmac239 gag induces siv-specific CD8 + t-cell responses in rhesus macaques . J. Virol., Apr 2010; 84: 3699 - 3706. CD8 t cell cross-reactivity networks mediate heterologous immunity in human ebv and murine vaccinia virus infections . J. Immunol., Mar 2010; 184: 2825 - 2838. diversity of the CD8 + t cell repertoire elicited against an immunodominant epitope does not depend on the context of infection . J. Immunol., Mar 2010; 184: 2958 - 2965.

225007536

NP_001139345.1

NM_001145873.1

P01732

25,729 Da

Adaptive Immunity Signaling Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); IL12-mediated Signaling Events Pathway (137922); Immune System Pathway (106386)

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: T cell receptor complex; integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; MHC class I protein binding; coreceptor activity; protein kinase binding. Biological Process: antigen processing and presentation; regulation of immune response; T cell activation; T cell mediated immunity; immune response; cytotoxic T cell differentiation; positive regulation of calcium-mediated signaling; defense response to virus; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Cd8 Deficiency, Familial

0.1 mg

280 USD

0.2 mg

385