catalog number :
MBS550708
products full name :
Anti-Human NT-4
products short name :
[NT-4]
other names :
[neurotrophin-4 preproprotein; neurotrophin-4; neurotrophin 4]
products gene name :
[NT-4]
other gene names :
[NTF4; NT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5]
purity :
The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
form :
Lyophilized from 0.2 filtered solution in PBS, pH 7.4.
storage stability :
The lyophilized antibody is stable for at least 3 years from date of receipt at -20°C. Upon reconstitution, this antibody can be stored in working alqiuots at 2-8°C for one month, or at -20°C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
tested application :
ELISA (EIA), Western Blot (WB)
app notes :
Dilution: A quick spin of the vial followed by reconstitution in H2O to a concentration of 1 mg/mL. Sandwich ELISA: This antibody can be used at 2-4 ug/mL to detect human NT4. The detection limit for recombinant human NT-4 is approximately at 2 ng/mL/ when used with cat#MBS553558 as a detection antibody. Western Blot: Use at a concentration range of 0.5- 2 ug/mL, the dtection limit for recombinant NT4 is a approximately 0.5-1 ng/lane under non-reducing conditions using compatible secondary reagents.
other info1 :
Host Note: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E.coli derived Human NT-4.
ncbi acc num :
NP_006170.1
ncbi gb acc num :
NM_006179.4
ncbi pathways :
Differentiation Pathway (1458184); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Neurotrophic Factor-mediated Trk Receptor Signaling Pathway (137977); Neurotrophin Signaling Pathway (101143); Neurotrophin Signaling Pathway (100064); P75(NTR)-mediated Signaling Pathway (138019)
ncbi summary :
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq, Jul 2008]