antibody

Monoclonal Mouse anti Human CD42b (gpIb)

MBS550103

0.5 mg

480 USD

monoclonal

mouse

nonconjugated

RKCD42

Antibody

Monoclonal Mouse anti Human CD42b (gpIb)

CD42b (gpIb)

Monoclonal Mouse anti Human CD42b; cd42; cd-42; monoclonal; mab; gpibB

Platelet glycoprotein Ib alpha chain; Platelet glycoprotein Ib alpha chain; Antigen CD42b-alpha

CD42

GP1BA; GP-Ib alpha; GPIb-alpha; GPIbA

GP1BA_HUMAN

Monoclonal

IgG1

RKCD42

Mouse

Human

626

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Western Blot: This monoclonal antibody has been tested in WB analysis of human CD42b. A suitable range of concentrations of this antibody for WB detection is 0.5-2 ug/ml.

Host Note: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human CD42b.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

Monoclonal

CD42b (gpIb) is part of a Glycoprotein Ib-IX-V (GPIb- IX-V) complex, the receptor for von Willebrand factor (VWF). This receptor plays a critical role in thrombus formation in damaged blood vessels under high shear. ssociation between VWF and GPIb-IX-V allows the adhesion protein to tether (or capture) rapidly flowing platelets into the developing thrombus. A fast off-rate of dissociation, however, means that platelets rapidly detach from VWF, unless integrins such as IIb3 or 21 are activated by intracellular signals, thereby enabling them to bind their ligands and mediate stable adhesion and thrombus growth.

biclonal (cd34+ CD42b +) myeloblastic and megakaryoblastic transformation of chronic myeloproliferative/myelodysplastic disorders . Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 5242.

121531

P07359.1

P07359

68,955 Da

Function: GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. Subunit structure: Two GP-Ib beta are disulfide-linked to one GP-Ib alpha. GP-IX is complexed with the GP-Ib heterodimer via a non covalent linkage. Interacts with FLNB. Ref.10 Ref.11 Ref.13. Subcellular location: Membrane; Single-pass type I membrane protein. Post-translational modification: Glycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. Polymorphism: Position 161 is associated with platelet-specific alloantigen Siba. Siba- has Thr-161 and Siba+ has Met-161. Siba is involved in neonatal alloimmune thrombocytopenia (NATP).Polymorphisms arise from a variable number of tandem 13-amino acid repeats of S-E-P-A-P-S-P-T-T-P-E-P-T in the mucin-like macroglycopeptide (Pro/Thr-rich) domain. Allele D (shown here) contains one repeat starting at position 415, allele C contains two repeats, allele B contains three repeats and allele A contains four repeats. Allele B is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy. Involvement in disease: Non-arteritic anterior ischemic optic neuropathy (NAION) [MIM:258660]: An ocular disease due to ischemic injury to the optic nerve. It usually affects the optic disk and leads to visual loss and optic disk swelling of a pallid nature. Visual loss is usually sudden, or over a few days at most and is usually permanent, with some recovery possibly occurring within the first weeks or months. Patients with small disks having smaller or non-existent cups have an anatomical predisposition for non-arteritic anterior ischemic optic neuropathy. As an ischemic episode evolves, the swelling compromises circulation, with a spiral of ischemia resulting in further neuronal damage.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.33Bernard-Soulier syndrome (BSS) [MIM:231200]: A coagulation disorder characterized by a prolonged bleeding time, unusually large platelets, thrombocytopenia, and impaired prothrombin consumption.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.19 Ref.21 Ref.22 Ref.27 Ref.28 Ref.31 Ref.32Bernard-Soulier syndrome A2, autosomal dominant (BSSA2) [MIM:153670]: A coagulation disorder characterized by mild to moderate bleeding tendency, thrombocytopenia, and an increased mean platelet volume. Some individuals have no symptoms. Mild bleeding tendencies manifest as epistaxis, gingival bleeding, menorrhagia, easy bruising, or prolonged bleeding after dental surgery.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.32Pseudo-von Willebrand disease (VWDP) [MIM:177820]: A bleeding disorder characterized by abnormally enhanced binding of von Willebrand factor by the platelet glycoprotein Ib (GP Ib) receptor complex. Hemostatic function is impaired due to the removal of VWF multimers from the circulation.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.23 Ref.24 Ref.25. Miscellaneous: Platelet activation apparently involves disruption of the macromolecular complex of GP-Ib with the platelet glycoprotein IX (GP-IX) and dissociation of GP-Ib from the actin-binding protein.Binding sites for vWF and thrombin (the latter site with unknown function) are in the N-terminal part of the molecule. Sequence similarities: Contains 7 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.Contains 1 LRRNT domain.

0.5 mg

480 USD