Monoclonal Mouse anti Human CD41 (gpIIb) | MyBioSource MBS550102 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Mouse anti Human CD41 (gpIIb)

catalog :

MBS550102

quantity :

0.5 mg

price :

480 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[RKCD41]

reactivity :

human

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS550102

products type :

Antibody

products full name :

Monoclonal Mouse anti Human CD41 (gpIIb)

products short name :

[CD41 (gpIIb)]

products name syn :

[Monoclonal Mouse anti Human CD41; cd41; cd-41; monoclonal; mab; integrin; gpiibB]

other names :

[integrin alpha-IIb preproprotein; Integrin alpha-IIb; GPalpha IIb; GPIIb; Platelet membrane glycoprotein IIb]

products gene name :

[CD41]

other gene names :

[ITGA2B; GP2B; ITGAB; GPIIb]

uniprot entry name :

ITA2B_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[RKCD41]

host :

Mouse

reactivity :

Human

sequence length :

1039

purity :

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

form :

Lyophilized from 0.2 um filtered solution in PBS pH 7.4.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20°C. Upon reconstitution, this antibody can be stored in working aliquots at 2°-8°C for one month, or at -20°C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.

app notes :

Flow Cytometry: This antibody can be used at 0.2 - 1 ug/10^6 cells. Western blot: Use at a concentration range of 0.5-2 ug/mL, the detection limit for recombinant SCF is approximately 2-4 ng/lane under non-reducing conditions using compatible secondary reagents. Dilution: A quick spin of the vial followed by reconstitution in H2O to a concentration of 1 mg/mL.

other info1 :

Host Note: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human SCF.

products categories :

Monoclonal

products description :

The platelet glycoprotein IIb ( CD41) constitutes the alpha subunit of a highly expressed platelet surface integrin protein. CD41 is a protein composed of two subunits that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Damage to blood vessels results in the release of a variety of intracellular mediators as well as initiation of the hemostatic cascade that in combination activate CD41/CD61 to bind to a variety of proteins including fibrinogen, fibronectin, von Willebrand factor and vitronectin.CD41/CD61 was once considered to be a platelet specific-receptor complex and has long been used as a phenotypic marker for the megakaryocyte- platelet lineage.

products references :

CD41 + progenitors are critical for the engraftment of hematopoietic cells derived from embryonic stem cells. . Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 2548. CD41 + cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis . Development, May 2008; 135: 1853 - 1862. modulation of murine embryonic stem cell derived CD41 +c-kit+ hematopoietic progenitors by ectopic expression of cdx genes . Blood, May 2008; 111: 4944 - 4953. analysis of thrombocyte development in CD41 -gfp transgenic zebrafish . Blood, Dec 2005; 106: 3803 - 3810. CD41 + and vascular endothelial (ve) -cadherin+ cells: two developmental origins of hematopoietic cell lineages that show different hematogenic potentials. . Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 3612.

ncbi gi num :

88758615

ncbi acc num :

NP_000410.2

ncbi gb acc num :

NM_000419.3

uniprot acc num :

P08514

ncbi mol weight :

113,377 Da

ncbi summary :

ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

uniprot summary :

ITGA2B: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. Belongs to the integrin alpha chain family. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3. Directly interacts with RNF181. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 17q21.32. Cellular Component: platelet alpha granule membrane; cell surface; focal adhesion; integral to plasma membrane; plasma membrane; integrin complex; external side of plasma membrane. Molecular Function: identical protein binding; protein binding; extracellular matrix binding; metal ion binding. Biological Process: integrin-mediated signaling pathway; axon guidance; platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; cell-matrix adhesion; positive regulation of leukocyte migration; blood coagulation; cell adhesion. Disease: Glanzmann Thrombasthenia; Bleeding Disorder, Platelet-type, 16

size1 :

0.5 mg

price1 :

480 USD

size2 :

1 mg

price2 :

775

more info or order :

MyBioSource product webpage