protein

Recombinant Human Cyclin-Dependent Kinase Inhibitor 2A, Isoform 1-TAT (rHuP16-INK4a-TAT)

MBS546348

0.005 mg

165 USD

Recombinant Protein

Recombinant Human Cyclin-Dependent Kinase Inhibitor 2A, Isoform 1-TAT (rHuP16-INK4a-TAT)

[p16-INK4a-TAT]

[p16-INK4a-TAT]

EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGRK KRRQRRR

> 95 % by SDS-PAGE and HPLC analyses

Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.0 .

This lyophilized preparation is stable at 2-8°C but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Source: E.coli. Molecular Weight: Approximately 18.0 kDa, a single non-glycosylated polypeptide chain containing 167 amino acids. Endotoxin: Less than 1 EU/ug of rHuP16-INK4a-TAT as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom . Reconstitute in ster ile distilled water or aqueous buffer containing 0 .1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solution s should be apportioned into working aliquots and stored at <=-20°C. Further dilutions should be made in appropriate buffered solutions.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Cyclin-dependent kindse inhibitors (CDKls) are proteins that bind to and inhibit the activity of CDKs. Two major classes of CDK inhi bito rs have been identified . The p16 family (pIS, p16, p18 and p19) binds to and inhi bits the activities of CDK4 and CDK6. The p21 family (p21, p27, p28 and p57) can bind to broad range of CDK-cyclin complexes and inhi bit t heir activities. CDKls are capable of su ppressing growth, and several lines of evidence strongly suggest t hat at least some CDKls may be tumor suppressor proteins. p16-INK4A is the member of pI6 fam ily and is encoded by CDKN2A gene in humans. It has three isoforms, which are wil d ly expressed but not detected in brain or skeletal muscle, except that isoform 3 is pancreasspecif ic. Defects in p161NK4A are a cause of Li-Fraumeni syndrome (LFS) and melanoma-astrocytomasyndrome (MASTS). The TAT transduction peptide can help rHuP16-INK4a with acrossing all kind of biomembranes in vivo and has no effect on the super structure of the protein.

0.005 mg

165 USD

0.025 mg

220

1 mg

2285