Recombinant Murine Interleukin?1 Alpha (rMuIL-1alpha) | MyBioSource MBS546295 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Murine Interleukin?1 Alpha (rMuIL-1alpha)

catalog :

MBS546295

quantity :

0.002 mg

price :

165 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS546295

products type :

Recombinant Protein

products full name :

Recombinant Murine Interleukin?1 Alpha (rMuIL-1alpha)

products short name :

[IL-1alpha]

other names :

[interleukin-1 alpha; Interleukin-1 alpha; interleukin-1 alpha; interleukin 1 alpha; Hematopoietin-1]

other gene names :

[IL1A; IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA; IL1F1; IL-1 alpha]

uniprot entry name :

IL1A_HUMAN

sequence length :

271

sequence :

SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS

purity :

> 97 % by SDS?PAGE and HPLC analyses.

form :

Lyophilized from a 0.2 ?m filtered solution in PBS, pH 7.4.

storage stability :

This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

other info1 :

Source: Escherichia coli. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 10^8 IU/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze/dried) powder.

other info2 :

Endotoxin: Less than 1 EU/ug of rMuIL-1Alpha as determined by LAL method.: Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < or = -20 °C. Further dilutions should be made in appropriate buffered solutions.

products description :

Murine Interleukin-1 alpha: Interleukin-1 alpha (IL-1Alpha) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1Alpha and IL-1Beta binds to the same receptor and has similar if not identical biological properties. Among various species, the amino acid sequence of mature IL-1Alpha s conserved 60 % to 70 % and human IL1 has been found to be biologically active on murine cell lines

ncbi gi num :

27894330

ncbi acc num :

NP_000566.3

ncbi gb acc num :

NM_000575.4

ncbi mol weight :

Approximately 17.9 kDa, a single non?glycosylated polypeptide chain containing 156 amino acids.

ncbi pathways :

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Cytokine Signaling In Immune System Pathway (1269310); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); FAS Pathway And Stress Induction Of HSP Regulation (198894); Graft-versus-host Disease Pathway (83124)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family. Protein type: Motility/polarity/chemotaxis; Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: cytosol; extracellular region; extracellular space. Molecular Function: copper ion binding; cytokine activity; interleukin-1 receptor binding; protein binding. Biological Process: apoptosis; cell proliferation; connective tissue replacement during inflammatory response; cytokine and chemokine mediated signaling pathway; fever; germ cell programmed cell death; immune response; inflammatory response; negative regulation of cell proliferation; positive regulation of angiogenesis; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; positive regulation of JNK cascade; positive regulation of mitosis; positive regulation of protein secretion; positive regulation of transcription from RNA polymerase II promoter; response to copper ion

size1 :

0.002 mg

price1 :

165 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

2755

more info or order :

MyBioSource product webpage