chemical

protein A

MBS546219

10 mg

135 USD

Biochemical

protein A

[Protein A]

The interaction between Protein A and IgG is not equivalent for all species.Even within a species, Protein A interacts with some subclasses of IgG and not others. For instance, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1.

>95% by SDS-PAGE analyses

Lyophilized with no additive.

2 years at -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

The Protein A molecule contains four high-affinity (K a =10 8 /mole) binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit (Table 1). Optimal binding occurs a pH 8.2, although binding is also good at neutral or physiological conditions (pH 7.0-7.6).

Source: Escherichia coli. Molecular Weight: Protein A is a cell wall component produced by several strains of Staphylococcusaureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series. It migrates with an apparent molecular mass of 45kDa in SDS-PAGE. A 280 of 0.1% solution: ~0.165 . Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Endotoxin: Less than 0.1EU/ug of Protein A as determined by LAL method. Reconstitution: Dissolve in distilled water or saline.

10 mg

135 USD

50 mg

200

250 mg

460

1 g

1115