catalog number :
MBS546215
products full name :
RecombinantMurineMCP-2/CCL8 (rMuMCP-2/CCL8)
products short name :
[MCP-2 (CCL8)]
products name syn :
[murine MCP-2 (CCL8); Murine MCP-2/CCL8]
products gene name :
[MCP-2 (CCL8)]
purity :
>97% by SDS-PAGE and HPLC analyses.
form :
Lyophilized from a 0.2 um filtered concentrated solution in 20mM PB, pH 7.4, 150 mM NaCI. Sterile filtered White Lyophilized (freeze-dried powder).
storage stability :
This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
other info1 :
MW Information: 8.5 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids and comprises only the chemokine domain of Human Fractalkine. AA Sequence: GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VFPTQKWVSE YMEILDQKSQ ILP.
other info2 :
Biological Activity: Fully biologically active when compared to standard. The ED 50 determined by a chemotaxis bioassay using human monoclytes is less than 100 ng/mL, corresoponding to a specific activity >1.0 x 10 4 IU/mg. Endotoxin Level: Less than 1EU/mg of rMuMCP-2/CCL8 as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20° C. Further dilutions should be made in appropriate buffered solutions.
products description :
MCP-2 and MCP-3 are two recently identified monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.