protein

Recombinant Tobacco Etch Virus Protease (rTEV)

MBS546186

300 IU

175 USD

Protein

Recombinant Tobacco Etch Virus Protease (rTEV)

[Tobacco Etch Virus Protease]

[Tobacco Etch Virus Protease; rTEV]

E Coli

Sterile FilteredWhite lyophilized (freeze-dried) powder.

Store rTEV at -70°C for long term or at -20°C for < 6 months.

Unit Definition: One unit of rTEV cleaves >=85% of 3 ug control substrate in 1 h at 30°C. buffer: 0.50 M Tris-HCl (pH 8.0), 10 mM DTT, 5 mM EDTA. Recommended Conditions for Cleavage of a Fusion protein: A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longerincubation times and/or more rTEV. Usage: This material is offered by Bio Basic Inc. for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

TEV Protease, Recombinant (rTEV) is a site-specific protease purified from E. coli by the affinity tag, GST tag. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30 degree C; however, the enzyme can be used at temperatures as low as 4 degree C. Following digestion, TEV Protease can be removed from the reaction via the GST tag sequence by affinity chromatography.

300 IU

175 USD

1000 IU

250

10000 IU

1020