protein

Recombinant Human Migration Inhibitor Factor (rHuMIF)

MBS546174

0.01 mg

165 USD

Protein

Recombinant Human Migration Inhibitor Factor (rHuMIF)

Migration Inhibitor Factor

Migration Inhibitor Factor; human MIF; Human Migration Inhibitory Factor

E Coli

>95% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. Physical Appearance: Sterile FilteredWhite lyophilized (freeze-dried) powder.

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Molecular Weight Information: Approximately 13 kDa, a monomeric, non-glycosylated polypeptide chain containing 117 amino acids.

Biological Activity: Fully biologically active measured by its ability to bind rhCD74 in a functional ELISA. Endotoxin Level: Less than 1EU/mg of rHuMIF as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

Human MIF consists of two á-helices and six -strands, four of which form a -sheet. The two remaining -strands interact with other MIF molecules, creating a trimer. Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position 1. Amino acids 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity. MIF has proinflammatory cytokine activity centered around aa's 49 - 65. On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates NO production and TNF-á release folllowing IFN- activation. MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction. Human MIF is active on mouse cells. Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine and rat MIF, respectively.

0.01 mg

165 USD

0.05 mg

205

1 mg

1945