protein

Recombinant Human Interleukin-1 alpha (rHu IL-1alpha)

MBS546158

0.002 mg

165 USD

Protein

Recombinant Human Interleukin-1 alpha (rHu IL-1alpha)

Interleukin-1 alpha

Interleukin-1 alpha; human IL-1alpha.; Human Interleukin-1 alpha; Interleukin-1 alpha; human IL-1a.; Recombinant Human Interleukin-1 alpha (rHu IL-1a)

interleukin 1 alpha

E Coli

268

>97% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.5. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine D10S cells is less then 0.001 ng/ml, corresponding to a Specific Activity of 1 x 109 IU/mg. Endotoxin Level: Less than 1EU/mg of rHuIL-1alpha as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

IL-1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.

300669780

BAJ11606.1

Approximately 18.0 kDa, a single nin-glycosylated polypeptide chain containing 159 amino acids.

0.002 mg

165 USD

0.01 mg

205

1 mg

2550