protein

Recombinant Human High-Mobility Group Box 1(His6-tagged) (rHu HMGB1)

MBS546104

0.01 mg

165 USD

Protein

Recombinant Human High-Mobility Group Box 1(His6-tagged) (rHu HMGB1)

HMGB1

human HMGB1; Human High-Mobility Group Box 1

high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1; high mobility group box 1; High mobility group protein 1

HMGB1

HMGB1; HMGB1; HMG1; HMG3; SBP-1; HMG1; HMG-1

HMGB1_HUMAN

E Coli

215

MAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFC5EYRPKIKGEHPGLSIGDVAKK LGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEK5KKKKEEEEDEE D EEDEE EEED EEDED E EEDD DDELEH H H H H H
MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFS
EFSKKCSERWKTMSAKEKGKFED

>95% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2 8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < = -20 degree C. Further dilutions should be made in appropriate buffered solutions.

Endotoxin Level: Less than 1EU/mg of rHuHMGB1 as determined by LAL method.

Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.

4504425

NP_002119.1

NM_002128.4

P09429

Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 233 amino acids.

Activated TLR4 Signalling Pathway (106400); Activation Of DNA Fragmentation Factor Pathway (105683); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Apoptosis Pathway (105648); Apoptosis Induced DNA Fragmentation Pathway (105682); Apoptotic Execution Phase Pathway (105677); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (833822)

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family. Protein type: DNA repair, damage; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus. Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; cytokine activity; damaged DNA binding; chromatin binding; transcription factor binding; transcription factor activity; DNA bending activity; single-stranded DNA binding; chemoattractant activity. Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; dendritic cell chemotaxis; DNA ligation during DNA repair; innate immune response; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding

0.01 mg

165 USD

0.05 mg

205

1 mg

1530