protein

Recombinant Human beta-Defensin 1 (rHu BD-1)

MBS546036

0.005 mg

165 USD

Protein

Recombinant Human beta-Defensin 1 (rHu BD-1)

[beta-Defensin 1]

[beta-Defensin 1; human BD-1 (47a.a); Human beta-Defensin 1]

[beta-defensin 1 preproprotein; Beta-defensin 1; beta-defensin 1; BD-1; beta-defensin-1; defensin, beta 1; Defensin, beta 1]

[DEFB1; DEFB1; BD1; HBD1; DEFB-1; DEFB101; BD1; HBD1; BD-1; hBD-1]

DEFB1_HUMAN

E Coli

68

>98% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 130mM NaCl. Physical Appearance: Sterile FilteredWhite lyophilized (freeze-dried) powder

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Host: E.coli. Molecular Weight Information: Approximately 5.0 KDa, a single non-glycosylated polypeptide chain containing 47 amino acids.

Biological Activity: Fully biologically active when compared to standard. Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1.0 ug/ml. Endotoxin Level: Less than 1EU/mg of rHuBD-1 as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta- defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues

4885181

NP_005209.1

NM_005218.3

P60022

Beta Defensins Pathway (530759); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]

defensin, beta 1: Has bactericidal activity. Belongs to the beta-defensin family. Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; Golgi lumen; extracellular region. Biological Process: G-protein coupled receptor protein signaling pathway; defense response to Gram-positive bacterium; innate immune response in mucosa; response to bacterium; antibacterial humoral response; innate immune response; immune response; response to testosterone stimulus; chemotaxis; acute inflammatory response

0.005 mg

165 USD

0.025 mg

205

1 mg

2050