catalog number :
MBS546028
products full name :
Recombinant Hepatitis B Surface Antigen preS1 (rHBsAg-preS1)
products short name :
[Hepatitis B Surface Antigen preS1]
products name syn :
[Hepatitis B Surface Antigen preS1; rHBsAg-preS1; Hepatitis B Surface Antigen preS1]
sequence :
MGGWSSKPRQGMGTNLSVPNPLGFFPDHQLDPAFGANSN
NPDWDFNPNKDHWPEAHQVGAGAFGPGFTPPHGGLLGWS
PQAQGILTTVPVAPPPASTNRQSGRQPTPISPPLRDSHP
QA
purity :
>95% by SDS-PAGE and HPLC analyses.
form :
Lyophilized from a 0.2um filtered concentrated (1mg/ml) soluditon in 20mM PB, pH7.4, 50mM NaCl. Physical Appearance: Sterile Filtered White lyohplized (freeze-dried) powder.
storage stability :
This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70 degree C. Avoid repeated freeze/thaw cycles.
tested application :
1. Immunochromatography (capture and conjugate). 2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS1. 3. ELISA
other info1 :
Molecular Weight Information: Approximately 12.3 kDa, a single non-glycosylated polypeptide chain containing 111 amino acids
other info2 :
Endotoxin Level: Less than 1EU/mg of rHBsAg-preS1 as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.
products description :
Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, LHBs has a 119 amino acid region called preS1.
ncbi mol weight :
12.6kDa, a single non-glycoslated polypeptide chain containing 119 amino acids.