chemical

PROTEINASE K (isolated from Tritirachium album)(fungal origin)

MBS545580

50 mg

145 USD

Biochemical

PROTEINASE K (isolated from Tritirachium album)(fungal origin)

[Proteinase K]

White lyophilized powder

Store at –20 degree C, though others have claimed stability at 2–8 degree C. When stored at –20 degree C, the product retains activity for about 3 years.

DNase, RNase and Protease: None-detected. Activity: >30 units/mg protein (hemoglobin, pH 7.5, 37 degree C). Unit definition: It is the amount of enzyme which releases at 37 degree C in 1 min as many folinpositive amino acid and peptides from hemoglobin as 1umol of tyrosine. Features: Proteinase K is a highlyactive and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.

Usage: In presence of 0.5-1.0% SDS Proteinase K inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids. Preparation Instructions: This product is soluble in double distilled water (1 mg/ml), yielding a clear colorless solution. Quality: Purified by chromatography and lyophilized. RNases: Not detectable. DNases: Not detectable. Exonucleases: Not detectable. Attention: Proteinase K can cause irritation of eyes, respiration tract and of skin. Proteinase K Activity in Commonly Used Buffers : Buffer: 20 mM Tris-HCl, pH 8.0 ;. Application Example: Reference ;. Activity (%): 100. Buffer: 10 mM Tris-HCl, 1 mM EDTA, 0.5 % SDS, pH 8.0;. Application Example: Bacterial genomic DNA isolation ;. Activity (%): 108. Buffer: 10 mM Tris-HCl, 100 mM NaCl, 25 mM EDTA, 1 % SDS, pH 8.0;. Application Example: Genomic DNA isolation from mammalian tissues ;. Activity (%): 171. Buffer: 100 mM Tris-HCl, 100 mM EDTA, 250 mM NaCl, 1% Sarkosyl, pH 8.0 ;. Application Example: Plant tissue genomic DNA isolation ;. Activity (%): 118. Buffer: 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 5 mM EDTA, 0.5 % SDS, pH 7.9 ;. Application Example: Inactivation of Calf Intestinal Alkaline Phosphatase ;. Activity (%): 104. Buffer: 50 mM Tris-HCl, 1 mM CaCl2, 3 mM DTT, 2 M Urea, pH 8.0 ;. Application Example: Denaturation of proteins ;. Activity (%): 66. Buffer: 10 mM Tris-HCl, 1.5 mM MgCl2, 50 mM KCl, 0.1 % Triton X-100, pH 8.8 ;. Application Example: PCR buffer ;. Activity (%): 158

50 mg

145 USD

250 mg

245

1 g

570