other

PERK Positive Control

MBS543108

5 Applications

290 USD

Positive Control

PERK Positive Control

[PERK]

[eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1; Eukaryotic translation initiation factor 2-alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; eukaryotic translation initiation factor 2 alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK]

[PERK]

[EIF2AK3; EIF2AK3; PEK; WRS; PERK; PEK; PERK; HsPEK]

E2AK3_HUMAN

1116

Western Blot Positive Control in ready to use SDS-sample buffer

Store at -20°C for long term storage

Western Blot (WB)

Western Blot: 1:500. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody.

Western Blot

Immunogen: Purified Protein. Alternative Nomenclature: DKFZp781H1925 antibody; E2AK3_HUMAN antibody; EC 2.7.11.1 antibody; Eif2ak3 antibody; Eukaryotic translation initiation factor 2 alpha kinase 3 antibody; Heme regulated EIF2 alpha kinase antibody; HRI antibody; HsPEK antibody; Pancreatic eIF2 alpha kinase antibody; PEK antibody; PRKR like endoplasmic reticulum kinase antibody; WRS antibody

Western Blot Positive Control Eukaryotic translation initiation factor 2-alpha kinase 3. Overview: Protein kinase- Eukaryotic translation initiation tactor 2-a kinase 3 (PERK) is a SerinelThreonine protein kinase, type I membrane protein of the endoplasmic reticulum that is abundant in secretory tissues and is inducible by ER-stress. ER stress increases the activity of PERK which phosphorylates the a subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, a reduction of translation initiation and repression of global protein synthesis., which then phosphorylates elF2a to promote reduced translation (1). PERK serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of Cyclin 01. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic 13 cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) which is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. PERK selective antibodies were generated against a peptide taken from the human PERK protein. The PERK-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage.

134304838

NP_004827

NM_004836.6

Q9NZJ5

134kDa

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Apoptosis Pathway (83060); Apoptosis Pathway (470); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873)

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK. Protein type: EC 2.7.11.1; Translation; Protein kinase, Other; Membrane protein, integral; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; PEK family; PEK subfamily. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm. Molecular Function: ATP binding; enzyme binding; eukaryotic translation initiation factor 2alpha kinase activity; Hsp90 protein binding; identical protein binding; protein binding; protein homodimerization activity; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity. Biological Process: angiogenesis; bone mineralization; calcium-mediated signaling; caspase activation; cellular response to amino acid starvation; cellular response to glucose starvation; chondrocyte development; endocrine pancreas development; endoplasmic reticulum organization and biogenesis; ER overload response; insulin secretion; insulin-like growth factor receptor signaling pathway; negative regulation of myelination; negative regulation of translation; negative regulation of translation initiation in response to stress; ossification; peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase I promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein homooligomerization; skeletal development; unfolded protein response. Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus

5 Applications

290 USD