Rabbit polyclonal Anti-Ki67 Antibody (C-epitope) | MyBioSource MBS541939 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit polyclonal Anti-Ki67 Antibody (C-epitope)

catalog :

MBS541939

quantity :

0.1 mg

price :

390 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS541939

products type :

Antibody

products full name :

Rabbit polyclonal Anti-Ki67 Antibody (C-epitope)

products short name :

[Ki67]

products name syn :

[Antigen identified by monoclonal antibody Ki 67; KIA; MKI67; Proliferation related Ki 67 antigen; RP11-380J17.2 antibody]

other names :

[antigen KI-67 isoform 1; Antigen KI-67; antigen KI-67; marker of proliferation Ki-67]

products gene name :

[Ki67]

other gene names :

[MKI67; MKI67; KIA; MIB-; MIB-1; PPP1R105]

uniprot entry name :

KI67_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

3256

specificity :

This antibody will detect both isoform 1 and isoform 2 in human

form :

Affinity Purified Immunoglobulins

concentration :

0.68mg/ml IgG in antibody stabilization buffer

storage stability :

-20 degree C for long term storage

tested application :

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

app notes :

DB: 1:10,000. ELISA:1:10,000. Immunoprecipitation: 1:200. Western Blot: 1:500

other info1 :

Immunogen: Synthetic peptide taken within amino acid region 2950-3000 on human antigen MBS541939 isoform 1. Expression: Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Determinant: Mid-region

other info2 :

Molecular Function: poly(A) RNA binding;protein C-terminus binding;ATP binding. Subcellular Location: Chromosome, Nucleus

products categories :

Primary Antibodies; Cell Biology; Cell Cycle; Ki67 Antibody

products description :

Ki-67 is a marker of cell proliferation and is detected in cells that are in G1-, S-, G2-, and M-phase of the cell cycle and absent in cells in GO phase (1). Ki-67 is used as a marker for cell proliferation of solid tumors and some hematological malignancies. It is associated with ribosomal RNA transcription, and inactivation of antigen Ki-67 leads to inhibition of ribosomal RNA synthesis. Ki-67 expression is used as a marker for determining the fraction of proliferating cells within a given population of tumor cells. It is also important for distinguishing between malignant and benign peripheral nerve sheath tumors. MBS541939 is used as prognostic/predictive markers in breast cancer and other malignant diseases, so high levels of MBS541939 are associated with poorer breast cancer survival (2). Ki-67 protein is a new member of the family of MPM-2 reactive phospho-proteins, which includes both structural and functional proteins that are necessary for the control and timing of mitosis. Phosphorylation and dephosphorylation of the Ki-67 protein are controlled by key regulatory structures of the cell cycle and occur at two hallmark events within the cell cycle: the breakdown and the reorganization of the nucleus during mitosis (3). The human Ki-67 gene maps to chromosome 1 Oq26.2 and encodes a 3256 amino acid protein.<br. The Ki-67 selective antibodies were generated against conserved sequences that are unique to specific Ki-67 protein. The selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. Western blot positive control (MBS542894) and antigeniC blocking peptides (MBS543898) for Ki67 are available.

ncbi gi num :

103472005

ncbi acc num :

NP_002408.3

ncbi gb acc num :

NM_002417.4

uniprot acc num :

P46013

ncbi summary :

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

uniprot summary :

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers. Protein type: Cell cycle regulation; Nucleolus. Chromosomal Location of Human Ortholog: 10q26.2. Cellular Component: chromosome, pericentric region; condensed chromosome; cytoplasm; membrane; nucleolus; nucleus. Molecular Function: ATP binding; protein binding; protein C-terminus binding. Biological Process: cell proliferation; DNA metabolic process; hyaluronan metabolic process; meiosis; organ regeneration; response to organic cyclic substance

size1 :

0.1 mg

price1 :

390 USD

more info or order :

MyBioSource product webpage