antibody

Na-dependent D-amino acid transporter 1 (N-epitope)

MBS540604

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunoprecipitation

Antibody

Na-dependent D-amino acid transporter 1 (N-epitope)

Na-dependent D-amino acid transporter 1

Anti-sodium-independent D-amino acid transporter Type 1 (ASC-1) antibodies; Affinity purified ASC-1Transporter Antibody

asc-type amino acid transporter 1; Asc-type amino acid transporter 1; asc-type amino acid transporter 1; solute carrier family 7 member 10; solute carrier family 7, (neutral amino acid transporter, y+ system) member 10; solute carrier family 7, (cationic amino acid transporter, y+ system) member 10; solute carrier family 7 (neutral amino acid transporter light chain, asc system), member 10; Solute carrier family 7 member 10

SLC7A10; SLC7A10; asc-1; HASC-1; ASC1; Asc-1

AAA1_HUMAN

Polyclonal

Rabbit

Human, mouse, rat

Affinity purifed

The antiserum is supplied in antibody stabilization buffer

~0.5mg/ml (IgG concentration 0.5-0.75 mg/ml.)

Shipped at 4 degree C. For long-term storage of antibody, store at -20 degree C MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer. Working solutions of antibodies in DiluOBuffer should be filtered through 0.45um filter after every use for long-term storage

Western Blot (WB), Immunoprecipitation (IP)

Antibody dilution 1:4,000 for ELISA or DOT blot assy. Antibody dilution 1:500-1,000 for WB using as sample. Immunoprecipitation: n.d; IHC n.d.

Immunogen: peptide . Reactivity Note: The antibody label 63-65 kDa ASC1 protein in PC-ASC1 sample

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8. Product Note: Briefly centrifuge to collect liquid, heat or boil PC-ASC1 tube for 1-2 minutes to dissolve any precipitate before use. This product is "ready-to-use" for electrophoresis. After thawing, keep at room temperature, Repeated freez/thaw may result in appearance of higher molecular weight immunoreactive bands

Sodium-Independent Amino Acid Transporter Antibodies

Amino acid transport across the plasma membrane is mediated by both sodium-dependent and sodium independent transport mechanisms (1). The recent advances in the molecular biology techniques let to discovery of several amino acid transporters that are homologues to the amino acid transport system describe for tissue culture cells and in membrane vesicle preparations (2). It has been shown that sodiumindependent amino acid transporter covalently interact with the heavy chain of a cell surface antigen 4F2 also known as CD98. Structurally, 4F2 compose of a 4F2 heavy chain (4F2hc), an integral membrane protein and a light chain that has intrinsic amino acid transport properties. Various amino acid transporters including transport type L, y+L and xc - has been shown to be the 2F4 light chains and assemble with 2F4hc for their functional amino acid transport activities (3-5). Recently a protein structurally related to amino acid transporter, with a characteristic 12 transmembrane domains (TMDs) has been cloned mouse brain that associates with 2F4hc for its small neutral amino acid transport activity (1). This transporter belongs to the LAT2 family, a component for system L transporter. The ASC-1 showed 65% identity to LAT2, 45% to LAT1, y+LAT1 and KIAA0245/y+LAT2 type transporters (6). The transporter ASC-1 is a member of the solute carrier family 7 and serves as a cationic amino acid transporter (y+ system). The ASC-1 protein has 530 amino acids (MW 63-64 kDa) is expressed in brain, lungs and intestine. It has two consensus phosphorylation sites (PKA and PKC) that are conserved in all y+ transport system. ASC1 transporter also transports with high affinity D and L-amino acid-related compounds and may involve in glutamenergic transmission in brain by modulating D-serine at glutamiergic synapse. ASC-1 forms disulfide dimmers with ASC transporter and SLC3A2/4F2hc proteins. The ASC-1-selective antibodies were generated against unique N and C-terminal peptides that are unique to ASC-1 protein. MyBioSource has generated specific rabbit anti-sodium independent amino acid transporter (ASC-1) polyclonal antibodies utilizing linear and cyclic peptide sequences. These antibodies have been fully characterized for cross reactivity with other members of the amino acid tranporter system. MyBioSource has produced antibodies to multiple epitopes on the same protein that will facilitate studies utilizing interspecies cross reactivity. MyBioSource provides ASC-1 Western blot positive control in "ready-to-use" SDS-PAGE sample buffer. The ASC-1 positive control appears as a diffuse band of 63-65 kDa and co-migrates with rat brain and lung sodium-independent amino acid transporter protein.

1. Christensen Physiological Rev. 70, 43-77; 1990. 2. Palacin M. et. al., J. Biol. Chem. 273, 23229-23632; 1998. 3. Pineda M. et. al., J. Biol. Chem. 274, 19738-19744; 1999. 4. Sato H. et. al., J. Biol. Chem. 274, 11455-11458; 1999. 5. Nakamura et. al., J. Biol. Chem. 274, 3009-3016; 1999. 6. Fukasawa et. al., J. Biol. Chem. 275, 9690-9698; 2000.

9790235

NP_062823.1

NM_019849.2

Q9NS82

56,798 Da

Amino Acid Transport Across The Plasma Membrane Pathway 106170!!Basigin Interactions Pathway 106065!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028!!SLC-mediated Transmembrane Transport Pathway 119558!!Transmembrane Transport Of Small Molecules Pathway 106572!!Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway 119559

SLC7A10, in association with 4F2HC (SLC3A2; MIM 158070), mediates high-affinity transport of D-serine and several other neutral amino acids (Nakauchi et al., 2000 [PubMed 10863037]).[supplied by OMIM, Mar 2008]

Function: Sodium-independent, high affinity transport of small neutral D- and L-amino acids. May play a role in the modulation of glutamatergic transmission through mobilization of D-serine at the glutamatergic synapse. Ref.1. Subunit structure: Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc . By similarity. UniProtKB Q9JMH8. Subcellular location: Membrane; Multi-pass membrane protein . Probable. Tissue specificity: Expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. Ref.1. Sequence similarities: Belongs to the amino acid-polyamine-organocation (APC) superfamily.

0.1-0.15 ml WB Control

290 USD