antibody

Melanocorticotropin hormone Receptor

MBS540415

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunoprecipitation

Antibody

Melanocorticotropin hormone Receptor

Melanocorticotropin hormone Receptor

Anti-Melanocortin Type 3 Receptor (MC3R) Antibodies (MC3R-301AP; MC3-312AP and MC3-321AP); Affinity purifeid MC3R antibodies (C-terminal)

melanocortin 1 receptor; Melanocyte-stimulating hormone receptor; melanocyte-stimulating hormone receptor; MC1-R; melanotropin receptor; melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocortin receptor 1

MC1R; MC1R; CMM5; MSH-R; SHEP2; MSHR; MSH-R; MC1-R

MSHR_HUMAN

Polyclonal

Rabbit

Human, mouse, rat

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with 0.02% sodium azide as preservative. The affinity-purified antibodies are isolated on immobilized antigen-affinity based chromatography. For long-term storage of antibodies, store at -20 degre

~0.5mg/ml (IgG concentration 0.5-0.6 mg/ml in antibody stabilization buffer)

Shipped at 4 degree C. Working solutions of antibodies in DiluOBuffer should be filtered through 0.45um filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

Antibodies are ideal for WB. Immunoprecipitation, IHC and other applications have not been standadrised. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay. WB: > 1:500; Immunoprecipitation & i.p pull-down assays: n.d

Immunogen: peptide . Reactivity Note: These antibodies detect a diffuse band at 55kDa MC3R protein in PC-MC3R samples. The antibody does not cross reacts with any other MC receptors

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

Melanocortin (MC) Receptor antibodies

The Proopiomelanocortin (POMC) is expressed in the arcuate nucleus of the hypothalamus (ARC) and the commissural nucleus of the solitary tract (cNTS) is post-translational processed to produce two melanocortin receptor ligands, alpha- and gamma-melanocyte-stimulating hormone (MSH). These endogenous peptides have long been reported to possess anti-inflammatory effects in many experimental models of acute and chronic inflammation, including experimental bowel disease, allergy, and chronic (mycobacterium-induced arthritis) and systemic inflammation (endotoxemia) (1). Melanocortins are ligands for a subgroup of GPCRs positively coupled to Adenylate cylcases, termed as melanocortin receptor (MC1-R, MC2R, MC3R, MC4R amd MC5R). These receptors are widely expressed at varied density throughout the body. Specific physioological actions have been implecated to specific MC recpetors such as skin pigmentation to MC1C, stimulation of adrenal steriodogenesis to MC2R, obesity ralted effects to MC4R and anti-inflammatory effects to MC3R (2) Two melanocortin receptors (MC3R, MC4R) are expressed in brain regions receiving projections of POMC fibers, most of which also receive projections from a population of arcuate neurons that co-express neuropeptide Y (NPY) and the MC3R/MC4R antagonist agouti-related peptide (AgRP). Recent dta suggest that MC3R knock out mice exhibit reduced linear growth and lean mass and are hyperleptinemic and hyperinsulinemic. The MC3R knock out mice did not show type 2 diabetes contract to a common finding in MC4R knock out mice (3). The Anti-MC3R-selective antibodies were generated against 3 unique epitopes from the MC3R receptor sequence that are conserved in several species. The peptides were post-synthetically modified to enhance antigenicty and conjugation to a carrier protien. The N-terminal, mid-refion and C-terminal epitopes are unique to MC3R receptor only. These antibodies do not cross react with other MC receptors. The polyclonal antibodies strongly labels a 55-60 kDa protein in PC-MC3R samples. Anti-MC3R-selective antibodies are also available in affinity-purified form for Immunoprecipitation, immunohistochemistry and Western blotting analyses. MyBioSource will also conjugate antibodies with fluorescent probes upon request at a nominal extra charge. MyBioSource, will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigenic peptides are also available. Please enquire for their availability before ordering.

1. Butler AA, Cone RD. Ann N Y Acad Sci. 2003 Jun;994:240-5. ). 2. Wikberg, J. E. S., R. Muceniece, I. Mandrika, P. Prusis, J. Lindblom, C. Post, A. Skottner. 2000. Pharmacol. Res. 42:393. 3. Stephen J. et. al., The Journal of Immunology, 2003, 170: 3323-3330. 4. Farooqui, S. M., Brock. W. J., A. Hamdi., Prasad. C. (1991) J. Neurochem. 57, 1363-1369.

5257476

AAD41351

Q01726

34,706 Da

Chaperonin-mediated Protein Folding Pathway 106242!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway 106243!!Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway 106245!!G Alpha (s) Signalling Events Pathway 119549!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!GPCRs, Class A Rhodopsin-like Pathway 198886!!Melanogenesis Pathway 83092!!Melanogenesis Pathway 504

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]

Function: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Subunit structure: Interacts with MGRN1, but does not undergo MGRN1-mediated ubiquitination; this interaction competes with GNAS-binding and thus inhibits agonist-induced cAMP production. Ref.14. Subcellular location: Cell membrane; Multi-pass membrane protein. Tissue specificity: Melanocytes and corticoadrenal tissue. Polymorphism: Genetic variants in MC1R define the skin/hair/eye pigmentation variation locus 2 (SHEP2) [. MIM:266300]. Hair, eye and skin pigmentation are among the most visible examples of human phenotypic variation, with a broad normal range that is subject to substantial geographic stratification. In the case of skin, individuals tend to have lighter pigmentation with increasing distance from the equator, with type I skin being the most lightly pigmented and type IV the most dark pigmented. By contrast, the majority of variation in human eye and hair color is found among individuals of European ancestry, with most other human populations fixed for brown eyes and black hair. Partial loss-of-function mutations are associated with fair skin, poor tanning and increased skin cancer risk.MC1R variants associated with red hair and fair skin, determine female-specific increased analgesia from kappa-opioid receptor agonist [. MIM:613098]. Involvement in disease: Melanoma, cutaneous malignant 5 (CMM5) [MIM:613099]: A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

0.1-0.15 ml WB Control

290 USD