antibody

Aquaporin 8 Antibody

MBS540353

0.1 mg

390 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, cow

western blot, ELISA, immunoprecipitation, enzyme immunoassay, dot blot

Antibody

Aquaporin 8 Antibody

[Aquaporin 8]

[AQP8; Aquaporin 8 antibody]

[aquaporin 8; Aquaporin-8; aquaporin-8; aquaporin 8]

[Aqp8; Aqp8; AQP-8; AQP-8]

AQP8_RAT

Polyclonal

Rabbit

Bovine, Human, Mouse, Rat, Sheep

263

Antigen Affinity Purified

Affinity Purified Immunoglobulins

0.65 mg/ml IgG in antibody stabilization buffer

Store at -20°C for long term storage

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), DOT Blot

ELISA: 1:10,000. Immunoprecipitation: 1:200. Western Blot: 1:500. DOT Blot: 1:10,000. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody.

Western Blot (WB)

Immunogen: Synthetic peptide taken within amino acid region 215-263 on rat Aquaporin 8 protein. Immunoglobulin: IgG. Expression: Sperm, pancreas and liver. Less expressed in salivary gland and absoptive colonic epithelial cells.

Molecular Function: Transport. Structure: Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala. Subcellular Location: Membrane; Multi-pass protein

Primary Antibodies; Channels & Transporters; Aquaporin 8 Antibody

Water channel proteins, known as Aquaporins, are transmembrane proteins that mediate osmotic water permeability. AQPs are a family of homologous water channels widely distributed in pla.nts, unicellular organisms, invertebrates, and vertebrates. Aquaporins are selective water/glycerol channels involved in the maintenance of volume homeostasis and ionic/osmotic balance. These water channels also enhance cellular tolerance against rapid freezing, which suggest that they might have some ecological relevance. Early work of Nobel Prize winner Peter Agre first cloned a 269 amino acid Aquaporin protein from red blood cell and kidney libraries that has six transmembrane domains (1). The amino terminal end of the protein was in the membrane and has two repeats that are at 1800 to each other and are responsible for water selectivity. The selective antibodies were generated against conserved sequences at the C-terminal end of the protein that is unique to rat/mouse Aquaporin 8 protein. The polyclonal antibodies were affinity purified on an immobilized antigen based affinity chromatography. The isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. Antigenic blocking peptides (MBS452379) and Western blot positive controls in ready to use SDS-sample buffers (MBS542541) are available.

2346968

BAA21918

P56405

28,055 Da

Aquaporin-mediated Transport Pathway (1376728); Bile Secretion Pathway (193141); Bile Secretion Pathway (193095); Passive Transport By Aquaporins Pathway (1376729); Transmembrane Transport Of Small Molecules Pathway (1376687)

plays a role in water transport across hepatocyte membranes [RGD, Feb 2006]

Forms a water-specific channel; mercury-sensitive. It may have an important role in spermatogenesis, in fertilization, and in the secretion of pancreatic juice and saliva.

0.1 mg

390 USD