antibody

5HT1A

MBS540293

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunoprecipitation

Antibody

5HT1A

5HT1A

Anti-5 Hydroxytryptamine 1A (5HT1A) receptor antibodies; Affinity purified 5HT1A antibodies

5-hydroxytryptamine receptor 1A; 5-hydroxytryptamine receptor 1A; 5-hydroxytryptamine receptor 1A; 5-HT1A; 5-HT-1A; serotonin receptor 1A; 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled; Serotonin receptor 1A

5HT1A

Htr1a; Htr1a; 5HT1A; RAT5HT1A; 5ht1a; 5-HT-1A; 5-HT1A

5HT1A_RAT

Polyclonal

Rabbit

Human, mouse, rat

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with preservatives. The affinity purified antibodies are isolated on antigen-affinity column.

~0.5mg/ml

Shipped at 4 degree C. For long-term storage, keep at -20 degree C. MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer. Working solutions of antibodies in DiluOBuffer should be filtered through 0.45um filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

Antibody dilution 1:50K for ELISA or DOT blot with PC-5HT1A. Antibody dilution 1:2.5K-5K for WB using 100 ug protein from rat striatal membranes. Immunoprecipitation, 4 ul of 5HT1A receptor antibody (5HT1A-101AP) will immunoprecipitate 65-75% 5HT1A receptor protein from solubilized COS1 cells transfected with 5HT1A cDNA. IHC; n.d

Immunogen: peptide (20mer). Epitope Location: 250-300. Reactivity Note: The dopamine D1 receptor antibodies label dopamine D1 receptor protein from COS1 cells transfected with dopamine D1 receptor cDNA as an average 45-47 kDa molecular weight protein.

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8. Product Note: Do not heat or boil PC-5HT1A samples. After thawing at room temperature, this product is "ready-to-use" for electrophoresis. Repeated freezing and thawing may result in appearance of higher molecular weight immunoreactive bands.

Serotonin (5Hydroxytryptamine) Receptor Antibodies

The serotonergic system is involved in both pathophysiology and treatment of addiction, depression or mood disorders, appetite control, cardiovascular function, motility of GI tract and sexual function. Serotonin (5-HT) has long been implicated in a number of neurodevelopmental processes including neuronal cell division, migration, neurite outgrowth, and synapse formation which regulate and control some of the above physiological manifestations. Molecular cloning of 5HT receptors have identified a family of closely related genes that encodes several proteins with extensive splice variants exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60 kDa. In brain the 5HT1A binding sites are tightly coupled to oestrous cycle and sex hormone levels (1). Serotonin receptors, also known as 5HT receptors are G-protein coupled rectors (7 transmembrane domains) that elicit the serotonin mediated effects on the above diverse physiological functions. There are at least 7 members of 5HT receptors (5HT1-5HT7), the 5HT1 is further divided in to 6 subtypes (5HT1A-5HT1F) each with multiple variants expressed as a results of alternate splicing or use of alternate initiation codons. Serotonin is reabsorbed by serotonin reuptake transporter which is coupled to sodium/calcium pump. 5HT1A receptors are grouped among the GPCR with characteristic 7TMD. There are several variants isoform of the 5HT1A receptors (5HT1A-5HT1E). The type if signal transduction could be excitatory or inhibitory depending upon the receptor functional coupling to the signal transduction complex. Certain beta arrestins are co-localized with 5HT receptors, where they act in concert with Bark1 or GRK1 and GRK 2 to regulate the expression of the GPCRs. All 5HT receptor proteins are glycosylated at or near the C-terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. 5HT receptors are believed to be associated with other proteins including members of the dopamine receptors and opiod receptors resulting in a significant increase in their apparent molecular weight. The 5HT receptor family-selective antibodies were generated against unique peptides selected form particular 5HT receptor isoform. MyBioSource has generated highly specific rabbit anti-5HT1A receptor polyclonal antibodies utilizing unique sequences. These antibodies have been fully characterized for cross reactivity with in the 5HT receptor family and with other cellular proteins. MyBioSource has produced antibodies to multiple epitopes on the same protein that will facilitate studies utilizing interspecies cross reactivity. MyBioSource provides 5HT1A Western blot positive control in "ready-to-use" SDS-PAGE sample buffer. The PC-5HT1A positive control appears as a diffuse band of 49-55 kDa and co-migrates with rat 5HT1A receptor protein on a 10% SDS-PAGE.

6981054

NP_036717

NM_012585.1

P19327

46,429 Da

GPCRs, Class A Rhodopsin-like Pathway 198500!!Monoamine GPCRs Pathway 198515!!Neuroactive Ligand-receptor Interaction Pathway 83445!!Neuroactive Ligand-receptor Interaction Pathway 462!!Serotonergic Synapse Pathway 525343!!Serotonin And Anxiety Pathway 755431

regulates AMPA receptor channels in the prefrontal cortex; regulation is mediated via inhibition of Ca2+/calmodulin-dependent kinase II [RGD, Feb 2006]

Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Subcellular location: Cell membrane; Multi-pass membrane protein. Sequence similarities: Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

0.1-0.15 ml WB Control

290 USD