Actinin 4 | MyBioSource MBS540264 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Actinin 4

catalog :

MBS540264

quantity :

0.1-0.15 ml WB Contr

price :

290 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS540264

products type :

Antibody

products full name :

Actinin 4

products short name :

Actinin 4

other names :

alpha-actinin-4; Alpha-actinin-4; alpha-actinin-4; actinin alpha4 isoform; non-muscle alpha-actinin 4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; actinin, alpha 4; F-actin cross-linking protein; Non-muscle alpha-actinin 4

other gene names :

ACTN4; ACTN4; FSGS; FSGS1; ACTININ-4

uniprot entry name :

ACTN4_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, mouse, rat

purity :

Affinity purifed

concentration :

~0.5mg/ml

storage stability :

Shipped at 4 degree C. Store at -20 degree C

tested application :

Western Blot (WB), Immunoprecipitation (IP)

other info1 :

Immunogen: peptide (17mer) . Epitope Location: 85-125

other info2 :

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

ncbi gi num :

12025678

ncbi acc num :

NP_004915

ncbi gb acc num :

NM_004924.4

uniprot acc num :

O43707

ncbi mol weight :

104,854 Da

ncbi pathways :

Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Arrhythmogenic Right Ventricular Cardiomyopathy Pathway 672454!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117293!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!Cell-Cell Communication Pathway 477132!!Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478

ncbi summary :

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Subunit structure: Homodimer; antiparallel . By similarity. Binds TRIM3 at the N-terminus . By similarity. Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 . By similarity. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with BAIAP1 and PDLIM2. Ref.7 Ref.8 Ref.9. Subcellular location: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7). Ref.9. Tissue specificity: Widely expressed. Involvement in disease: Focal segmental glomerulosclerosis 1 (FSGS1) [MIM:603278]: A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13. Sequence similarities: Belongs to the alpha-actinin family.Contains 1 actin-binding domain.Contains 2 CH (calponin-homology) domains.Contains 2 EF-hand domains.Contains 4 spectrin repeats. Sequence caution: The sequence AAC17470.1 differs from that shown. Reason: Frameshift at positions 19, 26, 124, 130, 589, 594, 787 and 806. The sequence BAA24447.1 differs from that shown. Reason: Erroneous initiation.

size :

0.1-0.15 ml WB Control

price :

290 USD

more info or order :

MyBioSource product webpage