antibody

Prominin 1 Antibody

MBS540104

0.1 mg Affinity-Puri

390 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Prominin 1 Antibody

Prominin 1

AC133; CD133; CORD12; Hematopoietic stem cell antigen; MCDR2; MSTP061; RP41; STGD4 antibody

PREDICTED: prominin-1 isoform X11; Prominin-1; prominin-1; prominin 1; Antigen AC133; Prominin-like protein 1; CD_antigen: CD133

PROM1; PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; PROML1

PROM1_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

787

Affinity Purified

0.50-0.95 ug/ul in antibody stabilization buffer

-20 degree C for long term storage

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Dot Blot: 1:2,000. ELISA: 1:2,000. Western Blot: 1:500-1:1,000

Immunogen: Synthetic peptide taken within amino acid regions 700-730 on CD133 protein. Expression: Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).

Molecular Function: Actinin binding; Cadherin binding. Structure: Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B. Subcellular Location: Apical cell membrane; Multi-pass membrane protein; Microvillus membrane; cilium; photoreceptor outer segment; Endoplasmic Reticulum-Golgi intermediate compartment

Primary Antibodies; Prominin 1 Antibody

May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).

578808417

XP_006714037.1

XM_006713974.3

94,605 Da

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell surface. Chromosomal Location of Human Ortholog: 4p15.32. Cellular Component: apical plasma membrane; cell surface; endoplasmic reticulum; ER-Golgi intermediate compartment; extracellular space; integral to plasma membrane; intracellular membrane-bound organelle; microvillus membrane; photoreceptor outer segment; plasma membrane; vesicle. Molecular Function: actinin binding; cadherin binding; protein binding. Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye. Disease: Cone-rod Dystrophy 12; Macular Dystrophy, Retinal, 2; Retinitis Pigmentosa 41; Stargardt Disease 4

0.1 mg Affinity-Purified

390 USD