antibody

Phospho-MERTK

MBS540099

0.1-0.15 ml WB Contr

415 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunoprecipitation

Antibody

Phospho-MERTK

Phospho-MERTK

Anti-Phospho-MERTK Antibodies (PMKT-140AP)

Human cellular proto-oncogene (c-mer) mRNA, complete cds; Tyrosine-protein kinase Mer; tyrosine-protein kinase Mer; STK kinase; proto-oncogene c-Mer; MER receptor tyrosine kinase; receptor tyrosine kinase MerTK; c-mer proto-oncogene tyrosine kinase; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK

MERTK

MERTK; MERTK; MER; RP38; c-mer; MER

MERTK_HUMAN

Polyclonal

Rabbit

Human, mouse, rat

Affinity purifed

The antisera are supplied in antibody stabilization buffer.

~0.5mg/ml (IgG concentration 0.65-1.25 mg/ml in 50% antibody stabilization buffer.)

Shipped at 4 degree C. For long-term storage of antibodies, store at -20 degree C. MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi-use antibody dilution buffer. Working solutions of antibodies in DiluOBuffer should be filtered through 0.45 um filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

Antibody is ideal for WB applications (Dilution for WB 1:750). The antibody has not been tested in other applications. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay.

Immunogen: peptide . Reactivity Note: This antibody detects only phosphorylated form of MERTK protein. The antibody does not have any reactivity towards nonphosphorylated protein or to other members of the tyrosine kinases.

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

Phospho-MER Receptor Tyrosine kinase (MERTK) selective antibodies

Tyrosine kinase activity is critical for transducing mitogenic signals inside the cells. Many of these tyrosine kinase proteins and receptors undergo auto-phosphorylation for the regulation of their activity (1, 2). Such autophosphorylation takes place at multiple sites and results in to phosphorylation of cellular substrates. The Nyk receptor tyrosine kinase was identified by screening human glioma expression library with an anti-Phosphotyrosine antibody. The same kinase was also independently identified and cloned by Graham and coworkers and they referred it as Mer (3). A chicken homologues for this gene is also identified as c-eyk (4). The Nyk/Mertk/c-eyk are the newest members of the Ufo/Axl family of receptor tyrosine kinases which are characterized by the presence of NCAM-like extra-cellular regions with 2 IgG-like domains juxtaposed with 2 fibronectin III-like regions. It is been shown that MERTK/Nyk has a strong mitogenic and transforming potential by activating PI 3 kinase, PLCgamma, MAP-kinase pathways (5). The auto-phosphorylation of MERTK was mapped on tryptic peptide containing amino acids 748-755 (CKKIY749SGDY753Y754RQG). The three tyrosine residues lie in the tyrosine kinase domain between sub-domains VII and VIII (6). It has been shown that kinase activity correlates with autophosphorylation of MERTK and that mutation of these tyrosine severely crippled the kinase activity (7). Autophosphorylation of this regions appears to be a common mechanism for several tyrosine kinases, however, the subtle differences in the pattern of phosphorylation was noted in various other kinase systems. It is also notes that preferable substrate for autophosphorylation in MERTK is the tyrosine 754 (7), substitution mutation to Phenylalanine exhibited a 90% decrease in atophosphorylation of MERTK compared to wild type protein. he receptor tyrosine kinase MERTK protein is a approximately 175-210 kDa protein. The MERTK protein has a tyrosine kinase domain closer to the C-terminal region and putative phosphorylation sites. The Anti-Phospho-MERTK-selective antibody was generated against phosphor-tyrosine containing peptide from a conserved sequence near the C-terminal end that is unique to MERTK/Nyk protein only. Phosphospecific antibodies were isolated on immobilized phosphor and non-phospho antigen affinity matrices. MyBioSource. also have Anti-MERTK selective antibodies as affinity-purified form for confocal, Western blotting and immunocytochemical analyses. MyBioSource will also conjugate antibodies with fluorescent probes upon request at extra charge. MyBioSource also provides antibodies against proteins that are involved in signal transduction, retinal degenerative diseases and against GPCRs. MyBioSource employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity. MyBioSource, will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available. Please enquire for their availability before ordering.

1. Kazlauskas A. Current Opin. Genet. Dev. 1994; 4; 5-14. 1. Heldin C. H. Cell 1995; 80; 213-223. 2. Graham D. K., Dawson T. L., Mullaney D. L., et. al., Cell Growth Diff. 1994; 5; 647-657. 3. Jia R. and Manafusa H. J. Biol. Chem. 1994; 269; 1839-1844. 4. Ling L. and Kung H. J. Mol. Cell Biol. 1995; 15; 6582-6592. 5. Hanks S. K. and Quinn A. M. Methods in Enzymology 1992; 200; 38-62. 6. Ling L., TempletonD and Kung H. J. J. Biol. Chem. 1996; 271; 18355-18362.

505664

U08023

Q12866

110,249 Da

Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028

This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008]

Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Ref.11. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Subunit structure: Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6 . By similarity. Interacts with VAV1 in a phosphotyrosine-independent manner. Ref.6 Ref.7 Ref.8 Ref.14. Subcellular location: Membrane; Single-pass type I membrane protein . By similarity. Tissue specificity: Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver. Post-translational modification: Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 . By similarity. Ref.5. Involvement in disease: Retinitis pigmentosa 38 (RP38) [MIM:613862]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.Contains 2 fibronectin type-III domains.Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.

0.1-0.15 ml WB Control

415 USD