antibody

Rabbit Polyclonal PGCC antibody

MBS540091

0.1 mg

425 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Rabbit Polyclonal PGCC antibody

[Natriuretic Receptor C / PGCC]

[ANPRC antibody, Atrial natriuretic peptide C type receptor antibody, C5orf23 antibody, FLJ14054 antibody, GUCY2B antibody, MGC22189 antibody, Natriuretic peptide receptor C/iguanylate cyclase C (atrionatriuretic peptide receptor C) antibody, Npr3 antibody, NPRC antibody.]

[guanylyl cyclase; Heat-stable enterotoxin receptor; heat-stable enterotoxin receptor; guanylate cyclase 2C; Guanylyl cyclase C; GC-C; Intestinal guanylate cyclase]

[PGCC]

[Gucy2c; Gucy2c; GCC; Guc2c; STA receptor; GC-C]

GUC2C_RAT

Polyclonal

Rabbit

Human, Mouse, Rat

1072

Affinity Purified Immunoglobulins

0.65 mg/ml IgG in antibody stabilization buffer

-20 degree C for long term storage

Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)

Confocal Microscopy: 1:200. Dot Blot: 1:10,000. ELISA: 1:10,000. Immunocytochemistry: 1:200. Immunofluorescence: 1:200. Immunohistochemistry: 1:200. Western Blot: 1:500

Western Blot (WB)

Immunofluorescence (IF)

Immunogen: Synthetic C-terminal peptide corresponding to unique amino acid sequences on Particulate Guanylyl Cyclase C protein.

Molecular Function: Lyase, Receptor. Structure: Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endoplasmic Reticulum membrane

Primary Antibodies; Signal Transduction; Second Messengers; cGMP; Natriuretic Peptide Receptor C / PGCC Antibody

Particulate Guanylate Cyclase C Antibody. The intracellular levels of Cyclic GMP (cGMP) are maintained by the activity of opposing enzymes: synthesizing gualylyl cyciases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate). While they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, soluble GC (sGC) contains a heme group and binds nitric oxide that activates the enzyme, while particulate GC (PGCs) are stimulated by natriuretic peptides. Particulate forms of guanylyl cyclases have been shown to function as natriuretic peptide receptors. In response to G-protein coupled receptor stimulation, the cGMP can be produced from GTP by either soluble guanylate cyclase (sGC), or by PGC. The sGC are heterodimers (a & b polypeptide chains), that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. There is significant structural homology among various PGCs: there is a large N-terminal extracellular domain(ECD), a single transmembrane domain, a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Guanylyl cyclase C (PGC-C), a member of membrane-bound guanylyl cyclases, is a receptor protein for guanylin and uroguanylin. The binding of a ligand to the extracellular domain of PGC-C triggers signal transduction, resulting in the regulation of intestinal fluids and electrolytes (1). A previous study proposed that a ligand-binding site on PGC-C is localized near the transmembrane region. The PGC-C type guanylate cyclase is a receptor for heat stable enterotoxin. PGC-C protein contains an extra-cellular amino acid sequence that is divergent from other PGCs, but retains several structural motifs (KLD, ECD and TMD). Disrupted activity of PGC-C may be involved in acute diarrhea. The Anti-PGC-C-selective antibodies were generated against conserved sequences near the C-terminal end of the protein that are unique to PGC-C protein. The PGC-C-selective antibodies are affinity purified against immobilized antigen based affinity chromatography that yielded epitope-specific antibodies. Antigenic blocking peptides (P-PGCC) and westem blot positive control (PC-PGCC) in rearly to use SDS-sample buffer are available. The PGC-C antibodies label a 127-130 kDa protein in various tissues including intestine kidney and spleen. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost.

548244

AAA41201.1

P23897

123,467 Da

Purine Metabolism Pathway (83337); Purine Metabolism Pathway (307)

guanylyl cyclase; functions as receptor for heat-stable enterotoxin responsible for acute diarrhea [RGD, Feb 2006]

GUCY2C: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin. Defects in GUCY2C are the cause of diarrhea type 6 (DIAR6). A relatively mild, early-onset chronic diarrhea that may be associated with increased susceptibility to inflammatory bowel disease, small-bowel obstruction, and esophagitis. Defects in GUCY2C are a cause of meconium ileus (MECIL). A condition characterized by a intestinal obstruction due to inspissated meconium in the distal ileum and cecum, which develops in utero and presents shortly after birth as a failure to pass meconium. Meconium ileus is a known clinical manifestation of cystic fibrosis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Protein type: Nucleotide Metabolism - purine; Lyase; Protein kinase, RGC; EC 4.6.1.2; Kinase, protein; Protein kinase, dual-specificity (receptor); Membrane protein, integral; Receptor, misc.; Guanylyl cyclase; RGC group; RGC family. Cellular Component: endoplasmic reticulum membrane; guanylate cyclase complex, soluble; integral to membrane; plasma membrane. Molecular Function: adenylate cyclase activity; ATP binding; GTP binding; guanylate cyclase activity; protein kinase activity; toxin binding. Biological Process: cGMP biosynthetic process; protein amino acid phosphorylation; regulation of cell proliferation; response to toxin; signal transduction

0.1 mg

425 USD