antibody

PDE6C

MBS540088

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, cow

western blot, immunoprecipitation

Antibody

PDE6C

PDE6C

Cone-specific cGMP-dependent phosphodiesterase Type 6C-alpha-subunit antibodies (PDE6C-101AP; FITC-PDE6C; P-PDE6C)

Pde6c protein; Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'; cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'; cGMP phosphodiesterase 6C; phosphodiesterase 6C, cGMP specific, cone, alpha prime; cGMP phosphodiesterase 6C

PDE6C

Pde6c; Pde6c; cpfl1

PDE6C_MOUSE

Polyclonal

Rabbit

Human, mouse, rat, bovine

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with 0.02% sodium azide. The affinity-purified antibodies are purified on antigenspeharose affinity column and supplied as 0.5-1.25 mg/ml IgG in antibody stabilization buffer.

~0.5mg/ml (Ig concentration is in the range of 0.73-0.75mg/ml in antibody stabilization buffer.)

Shipped at 4 degree C. For long-term storage of antibodies, store at - 20 degree C. MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in our multi-use antibody dilution buffer (DiluOBuffer). Working solutions of antibodies in DiluOBuffer should be filtered through 0.45um filter after every use for long-term storage

Western Blot (WB), Immunoprecipitation (IP)

Antibody is ideal for WB. The dilutions for this antibody is for reference only, investigators are expected to determine optimal conditions for specific assays. WB 1:500, Immunoprecipitation & i.p pull-down assays: n.d

Immunogen: peptide . Reactivity Note: This antibody detects a 103 kDa PDE6C subunit proteins.

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

cGMP-dependent phosphodiesterase type-6 (PDE6) selective antibodies

Cone-specific cGMP-dependent phosphodiesterase Type 6C-alpha-subunit antibodies (PDE6C-101AP, FITC-PDE6C, P-PDE6C) The retinal cone and rod photoreceptor cells can directly stimulate and response by photons of the light. Upon light stimulation these cells undergo a highly regulated cascade of events that leads to changes in the photoreceptor membrane potentials and subsequent neurotransmitterrelease. The key component of photo transduction in retina is retinal specific cGMP-dependent phosphodiesterases type 6 (PDE6s). These PDEs convert cGMP in to GMP when activated by the G-protein transducin and caused a closer of the cGMP gated ion channels, reduced Na+ and Ca++ influx and hyper polarization of the photoreceptor cells. Rodhopsin and PDE6 are also co-localized in the internal membraneous disk of the photoreceptor cells allowing most efficient transfer of light signal from one protein to other. The retinal phosphodiesterses are multimeric proteins consisting of catalytic and inhibitory subunits. The large alpha and beta (and) subunits of the rod and cone specific PDE6 dimerises to form the catalytic complex of the PDE6 isozyme. The smaller gamma subunit serves as an inhibitor for the rod and cone phosphodiesterses (1). There are 3 subtypes of PDE6 found in rod and cone cells, PDE6 PDE6 and an inhibitory PDE6. The rod and cone PDEs differ significantly in subunit compositions, sensitivity to transducin, and cGP binding properties (2). The PDE6 and forms have 2 non catalytic binding cGMP sites; the cone biding site has significantly lower Ki for cGMP that the rod enzymes. There is significant homology between a and b subunits in the catalytic and GAF binding domains. Both PDE6 and PDE6 are post-translationally modified, PDE6 is farnsylated and beta subunit is gernylgernylated (3). Generally, these modifications will allow proteins to be anchored to the membranes, incase of PDE6, despite 2 lipid modifications the PDE can easily be removed form membranes by detergent free hypotonic buffers. The PDE6 can be localized in both membrane and cytosolic compartment, the cytosolic PDE6 has been show to be associated with a 17 kDa delta subunit (4). The role of delta subunit in PDE6 functioning is not fully understood. It been hypothesized that delta subunit might be playing a role in PDE6 solubility in the photoreceptor cells. The PDE6 is a 103- 105 kDa protein (858 amino acids) with 2 GAF and one PDEase catalytic domain. MyBioSource has made PDE6C-selective antibodies against a peptide sequence taken form region between GAF and the PDE catalytic domain. PDE6C antibodies are raised in rabbits and are affinity purified by immobilized-antigen-based affinity chromatography. MyBioSource. also provides antibodies to all PDEs and against various PDE6 subtypes and variants. MyBioSource. also carries a number antibodies to PDE families, family and subtype-selective and family-subtype-variant selective for detailed analyses of cyclic nucleotide signaling pathways. The PDE6C-selective antibodies (PDE6C-101AP) are generated against peptides from unique sequence on the cone specific PDE6C gene. The affinity-purified antibody (PDE6C-101AP) labels 104kDa PDE6C protein in our WB positive control for PDE6C (PC-PDE6C). The PDE6C-specific antiserum has no cross reactivity against other PDE6 subunits or other PDE family members. PDE6C-selective antibody is also available in affinity purified form for confocal, WB & IHC analyses.MyBioSource will also conjugate antibodies with enzyme.

1. Florio S. K., Prusti R.K., and Beavo J. A., J. Biol. Chem. 271; 24036-24047; 1996. 2. Charbonneau H., Prusti R. K., et al., Proc. Natl. Aad. Sci. USA 87; 288-292; 1990. 3. Cook T. A., Ghomashchi F., Gelb M. H., et. al., J. Biol. Chem. October 26, 2000. 4. Gillespie P. G., and Beavo J. A. J. Biol. Chem. 263; 8133-8141; 1988.

20071906

AAH27050.1

Q91ZQ1

98,785 Da

Purine Metabolism Pathway 83144!!Purine Metabolism Pathway 672434!!Purine Metabolism Pathway 307!!Cyclic GMP Biosynthesis Pathway 542873

Catalytic activity: Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. Cofactor: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions . By similarity. Subunit structure: Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa . By similarity. Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side . Potential. Sequence similarities: Belongs to the cyclic nucleotide phosphodiesterase family.Contains 2 GAF domains.

0.1-0.15 ml WB Control

290 USD