antibody

PDE10

MBS540056

0.1-0.15 ml WB Contr

415 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunoprecipitation

Antibody

PDE10

PDE10

cAMP-dependent phosphodiesterase Type 10A-antibodies; Affinity Purified

phosphodiesterase 10A1 (PDE10A1); cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; phosphodiesterase 10A1 (PDE10A1); dJ416F21.1 (phosphodiesterase 10A); phosphodiesterase 10A

PDE10

PDE10A; PDE10A; HSPDE10A

PDE10_HUMAN

Polyclonal

Rabbit

Human, mouse, rat, monkey

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with 0.02% thimerosal or merthiolate. The affinity-purified antibodies are purified on antigen-speharose affinity column and supplied as 0.6-0.75 mg/ml IgG in antibody stabilization buffer contain

~0.5mg/ml (IgG concentration 0.7-0.75 mg/ml in antibody stabilization buffer.)

Shipped at 4 degree C. Working solutions of antibodies in DiluOBuffer should be filtered through 0.45 um filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

Antibody is ideal for IP/ PDE10 activity measurement and for IHC. Other applications are not tested. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay in his/her laboratory. WB > 1:500 ; IP & i.p pull-down assays: > 1:250

Immunogen: peptide . Reactivity Note: This antibody detects a 90-93kDa PDE10A proteins from rat brain and pancreas.

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

cAMP-dependent phosphodiesterase type-10A (PDE10A) selective antibodies

Cyclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP and cGMP in to AMP and GMP respectively are phosphodiesterase type 10. Only one member of the cAMP and cGMP-specific phosphodiesterase type-10 (PDE10) family is cloned and characterized. The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and or use of alternate initiation sites (1). The PDE10 gene was first cloned from fetal human lung tissue library using bioinformatics approach (2). The deduced amino acid sequence contains 779 amino acids (MW 93-95 kDa) including a putative cGMP binding domain near the N-terminal region of the protein. The catalytic portion is 16-47% identical to other PDEs and PDE10 hydrolyze cAMP and cGMP with Km 0.26 and 7.2 uM with Vmax for cGMP twice as much that for cAMP. The presence of cAMP inhibits the hydrolysis of cGMP and vise versa. PDE10A is widely expressed in putamen and caudate nucleus in the brain, thyroid and testis. Surprisingly the expression of PDE10A was also abundant in Heart (see figure 1). MyBioSource also provides antibodies to other PDE family members. The PDE-selective antibodies are also available for family-subtypeselective and family-subtype-variant selective antibodies for detailed analyses of cyclic nucleotide signaling pathways. The PDE10A-selective antibodies (PD10A-100P and PD10A-101AP) are generated against a common sequence near the 250-325 amino acids down stream from Nterminus. The multiple antigenic peptide (MAP) was generated using multiple antigen peptide resin. The polyclonal antibody (PD10A- 100P/101AP) labels a 93 and 95 kDa PDE10A1 and PDE10A2 variants in striatum, testis and pancreas. The PDE10A-specific antiserum has no cross reactivity against any other PDE family members tested so far.

1. Kotera J., Fugishge K., Yuasa K., Omari K. Bio. Chem. Biophys. Res. Comm. 261, 551-557, 1999. 2. Fujishige, K., Kotera J., Omori K. Eur. J. Biochem. 266, 1118-1127, 1999.

10716139

BAB16383

Q9Y233

88,412 Da

G Alpha (s) Signalling Events Pathway 119549!!GPCR Downstream Signaling Pathway 119548!!Hemostasis Pathway 106028!!Morphine Addiction Pathway 552665!!Morphine Addiction Pathway 554808!!Nitric Oxide Stimulates Guanylate Cyclase Pathway 187200!!Platelet Homeostasis Pathway 187198!!Purine Metabolism Pathway 82944!!Purine Metabolism Pathway 307!!Signal Transduction Pathway 477114

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2011]

Function: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. Ref.11. Catalytic activity: Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. Cofactor: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. Ref.11. Enzyme regulation: Inhibited by dipyridamole and moderately by IBMX. cAMP acts as an allosteric activator. Ref.8. Pathway: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. Subunit structure: Homodimer. Ref.12. Subcellular location: Cytoplasm. Note: Located mostly to soluble cellular fractions. Tissue specificity: Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum. Domain: The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity. Ref.8 Ref.12Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region. Ref.8 Ref.12. Sequence similarities: Belongs to the cyclic nucleotide phosphodiesterase family.Contains 2 GAF domains. Biophysicochemical propertiesKinetic parameters:KM=56 nM for cAMP Ref.11KM=4.4 uM for cGMPVmax=507 nmol/min/mg enzyme for cAMPVmax=1860 nmol/min/mg enzyme for cGMP. Sequence caution: The sequence AAD32596.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.1-0.15 ml WB Control

415 USD