antibody

Adenylate Cyclase 9 (AC9)

MBS540051

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

Antibody

Adenylate Cyclase 9 (AC9)

Adenylate Cyclase 9 (AC9)

Anti-Aquoprin 3Antibodies (AQP3-301AP)

adenylate cyclase type 9; Adenylate cyclase type 9; adenylate cyclase type 9; adenylyl cyclase 9; ATP pyrophosphate-lyase 9; adenylate cyclase type IX; type IX ATP pyrophosphate-lyase; adenylate cyclase 9; ATP pyrophosphate-lyase 9; Adenylate cyclase type IX; Adenylyl cyclase 9

AC9

ADCY9; ADCY9; AC9; KIAA0520

ADCY9_HUMAN

Polyclonal

Rabbit

Affinity purifed

~0.5mg/ml (0.50-0.95 mg/ml of antibody stabilization buffer)

Shipped at 4 degree C. Store at -20 degree C

Western Blot (WB), Immunoprecipitation (IP)

Is tested for WB application at 1:500 dilution. Other applications for this antibody has not been tested. WB: > 1:500; Immunoprecipitation & i.p pull-down assays: n.d; IHC n.d (Antibody dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions).

Immunogen: peptide

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

Antibodies to Water channel/glycerol channel protiens

Water channel proteins, known as aquaporins, are transmembrane proteins that mediate osmotic water permeability. The discovery of aquaporin (AQP) has made a great impact on life sciences. AQPs are a family of homologous water channels widely distributed in plants, unicellular organisms, invertebrates, and vertebrates. Aquaporins are selective water/glycerol channels involved in the maintenance of volume homeostasis and ionic/osmotic balance. These water channels also enhance cellular tolerance against rapid freezing, which suggest that they might have some ecological relevance also. Early work of Nobel Prize winner, Peter Agre and his group first clone a 269 amio acid protein from RBC and kidney libraries that has a 6 TMD (1). The amino terminal end of the protein was in the membrane and has 2 repeats that are at 180oto each other and are responsible for water selectivity. Now there are atlest 12 isoforms of Aquaporins area cloned. Acetazolamide can bind to Aquaporin 1 and inhibit the water movement across the plasma membrane. In brain, three main AQPs AQP1, AQP4 and AQP9 are expressed and are implicated in numerous physiological functions. AQP1 has a role in cerebrospinal fluid formation, whereas AQP4 is involved in water homeostasis and extracellular osmotic pressure in brain parenchyma. AQP4 seems also to have an important function in oedema formation after brain trauma or brain ischemia. AQP9 is implicated in brain energy metabolism (2). AQP1 and 4 are also implicated in water balance in retina and other eye components. Studies also suggest that AQP1 may be responsible for the high vascular permeability and interstitial fluid pressure in tumors of the brain, colon, breast and pancreas. AQP1 may also play a role in tumor angiogenesis and in the development of effusions or edema fluid. The Anti-AQP3 selective antibodies were generated against conserved sequences at or near C-terminal end of the protein that is unique to rat/mouse aquaporin 3 protein. The AQP3-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The APQ3 antibodies label a 29-32 kDa protein in Western blot using Western blot positive control for AQP3 (PC-AQP3). Anti-AQP3-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses. MyBioSource will also conjugate antibodies with fluorescent probes upon request at extra charge. Limited quantities of antigenic peptide is also available (inquire before ordering). MyBioSource also provides antibodies to other family members of aquaporins and aquaglyceroporins including AQP1-AQP12. MyBioSource employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (6). MyBioSource will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available for blocking studies. Please enquire for their availability before ordering.

1. Peter Agre The Aquaporin Water Channels. Proc. Thorac. Soc. 3, 5-13, 2006. 2. Guerin CF, Regli L, Badaut J. [Roles of aquaporins in the brain] Med Sci (Paris). 2005 Aug-Sep;21(8-9):747-52.

50959205

NP_001107.2

NM_001116.3

O60503

150,701 Da

Activation Of GABAB Receptors Pathway 187181!!Adenylate Cyclase Activating Pathway 106499!!Adenylate Cyclase Inhibitory Pathway 106500!!Aquaporin-mediated Transport Pathway 187187!!Bile Secretion Pathway 193146!!Bile Secretion Pathway 193095!!Ca-dependent Events Pathway 106497!!CaM Pathway 106468!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Calcium Signaling Pathway 83050

Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. It is regulated by a family of G protein-coupled receptors, protein kinases, and calcium. The type 9 adenylyl cyclase is a widely distributed adenylyl cyclase, and it is stimulated by beta-adrenergic receptor activation but is insensitive to forskolin, calcium, and somatostatin. [provided by RefSeq, Jul 2008]

Function: May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin . By similarity. Catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate. Cofactor: Binds 2 magnesium ions per subunit. Enzyme regulation: Insensitive to calcium/calmodulin, forskolin and somatostatin. Stimulated by beta-adrenergic receptor activation. Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity: Expressed in multiple cells of the lung, with expression highest in airway smooth muscle. Ref.2. Sequence similarities: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.Contains 2 guanylate cyclase domains. Sequence caution: The sequence AAC24201.1 differs from that shown. Reason: Frameshift at position 1252. The sequence BAA25446.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.1-0.15 ml WB Control

290 USD