antibody

Purinergic Receptor P2Y2

MBS540048

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

Antibody

Purinergic Receptor P2Y2

Purinergic Receptor P2Y2

Anti-P2Y2 nucleotide Receptor 2 (P2Y2R) Antibodies (P2Y2-201AP)

purinergic receptor P2Y, G-protein coupled, 2, isoform CRA_a; P2Y purinoceptor 2; P2Y purinoceptor 2; ATP receptor; P2U receptor 1; purinoceptor P2Y2; P2U purinoceptor 1; P2U nucleotide receptor; purinergic receptor P2Y2; purinergic receptor P2Y, G-protein coupled, 2; ATP receptor; P2U purinoceptor 1; P2U1; P2U receptor 1; Purinergic receptor

P2RY2; P2RY2; P2U; HP2U; P2U1; P2UR; P2Y2; P2RU1; P2Y2R; P2RU1; P2Y2; P2U1

P2RY2_HUMAN

Polyclonal

Rabbit

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with 0.02% sodium azide as preservative. For long-term storage of antibodies, store at -20 degree C. MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepar

~0.5mg/ml (IgG concentration 0.52-0.68mg/ml in antibody stabilization buffer.)

Shipped at 4 degree C. Working solutions of antibodies in DiluOBuffer should be filtered through 0.45um filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

Antibody is ideal for WB and ELISA assays. Application in IHC, confocal and immpunoprecipitation protocol is not yet characterized. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay. WB > 1:500; Immunoprecipitation & i.p pull-down assays: n.d; IHC, n.d. Application of this antibody in protocols not listed here does not necessarily exclude its use in such procedures.

Immunogen: peptide . Reactivity Note: This antibody detects a single 56-68kDa P2Y2 diffused band in PC-P2Y2 samples. The antibody does not cross reacts with other P2X or P2Y receptor members on western blots.

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

Purinergic Receptor selective antibodies

Purinenucletide receptor are classified in to two classes, the ionotrophic ligand-gated channels, P2X and the metabotropic G-protein coupled receptors, P2Y (1). There are 7 P2X receptors and 11 P2Y (P2Y1-2, 4, 6 and 11-14) each with unique agonist response profile has been identified. The subtypes of metabotropic receptors P2Y1 and P2Y2 are predominantly expressed in arterinal smooth muscle. The activation nof P2Y2 receptors led to release of intracellular calcium by phospholipase C action after which receptor phosphorylation appears to regulate desensitization of the P2Y2 receptor via action of protein kinase C. The purinergic receptors P2Y2 couples to the activation of mitogen activated protein kinases (MAPK) and mobilize the intracellular calcium stores, activation of phospholipase Cb, protein ikinase C isofroms, focal adhesion kinases and c-Src kinases. These pathways are implicated in number of physiological and pathological processes including inflammation, neuroprotection, active astrogliosis etc (1, 2). P2Y2 receptor induce chloride secretion in airway epithelial cells independent of cystic fibrosis transmembrane conductance regulator and this receptor ligands are therapeutically important for the treatment of retinal detachment and improved mucociliary clearance in cystic fibrosis patients. The P2Y2 nucleotide receptor is seven TMD GPCR family member and is coupled to a Gq protein that is stimulated equipotently by UTP and ATP, mediating activation of phospholipase C-beta (PLC-) and mitogen-activated protein kinase (MAPK). The P2y2 undergoes rapid agonist induce desensitization and phosphorylation at Serine 243, threonine 344 and serine 356 (1). The antagonist potencies for P2Y2 receptor is in the order of suramin>>PPADS= RB-2>TNPATP and suramin (pA2, 5.40) was a competitive antagonist (3). The extracellular acidification does not affect the ATP and UTP potencies of P2Y2 receptors while the P2Y4 receptor potencies are increased by 8-10-fold (3). Both P2Y2 and P2Y4 receptors regulate the UTP induced chloride secretion from intestinal mucosa and cuased endothelium induced relaxation in aorta (4, 5). P2Y2 receptor when expressed in host cells showed significant heterogeneity on SDS-PAGE mobility like many other PGCRs (6). The P2Y2 is a 377 aa protein with characteristic 7TMDs of GPCR, exhibit a broad band od 52-76 kDa (7). The P2Y receptor sequence analyses suggest the presence of putative Nglycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins and has several phosphorylation sites. The Anti-P2Y2-selective antibodies were generated against conserved but unique sequences on from P2Y2 gene that are expressed only in P2Y2 and was common in several other species. The polyclonal antibodies were affinity purified on an immobilized antigen based affinity chromatography. These epitope-spcific antibodies strongly labeled P2Y2 protein form P2Y2-western blot positive controls. Anti-P2Y2-selective antibodies are also available as fluorescent conjugates for direct application in IHC. MyBioSource, will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigenic peptides are also available. Please enquire for their availability before ordering.

1. Abbracchio, M., and G. Burnstock. 1994. Purinoceptors: are there families of P2X and P2Y purinoceptors Pharmacol. Ther. 64:445-475. 2. Rosa V. Flores,1 Melvin G. Hernández-Pérez,1 Edna Aquino,1§ Richard C. Garrad,3 Gary A. Weisman,4 and Fernando A. Gonzalez1,2*Mol Cell Biochem. Agonist-induced Phosphorylation and Desensitization of the P2Y2 Nucleotide Receptor. 2005 December; 280(1-2): 35-45. 3. Scott S Wildman, Robert J Unwin, and Brian F King., Extended pharmacological profiles of rat P2Y2 and rat P2Y4 receptors and their sensitivity to extracellular H+ and Zn2+ ions. Br J Pharmacol. 2003 December; 140(7): 1177-1186. 4. Esam Ghanem, Bernard Robaye, Teresinha Leal, Jens Leipziger, Willy Van Driessche, Renaud Beauwens, and Jean-Marie Boeynaems. The role of epithelial P2Y2 and P2Y4 receptors in the regulation of intestinal chloride secretion. Br J Pharmacol. 2005 October; 146(3): 364-369. 5. Pieter-Jan D F Guns, Tim Van Assche, Paul Fransen, Bernard Robaye, Jean-Marie Boeynaems, and Hidde Bult. Endothelium-dependent relaxation evoked by ATP and UTP in the aorta of P2Y2-deficient mice. Br J Pharmacol. 2006 March; 147(5): 569-574. 6. Rosa V. Flores, Melvin G. Hernández-Pérez, Edna Aquino, Richard C. Garrad, Gary A. Weisman, and Fernando A. Gonzalez. Agonist-induced Phosphorylation and Desensitization of the P2Y2 Nucleotide Receptor. Mol Cell Biochem. Author manuscript; available in PMC 2006 November Farooqui et. al.,

119595289

EAW74883

P41231

42,273 Da

Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!G Alpha (q) Signalling Events Pathway 106043!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!GPCRs, Class A Rhodopsin-like Pathway 198886!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 645295!!Neuroactive Ligand-receptor Interaction Pathway 83053!!Neuroactive Ligand-receptor Interaction Pathway 462!!Nucleotide GPCRs Pathway 198869!!Nucleotide-like (purinergic) Receptors Pathway 106373

The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Mar 2013]

Function: Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP. Subcellular location: Cell membrane; Multi-pass membrane protein. Tissue specificity: Spleen, testis, kidney, liver, lung, heart and brain. Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

0.1-0.15 ml WB Control

290 USD