antibody

Norepinephrine Transporter 1

MBS540046

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

Antibody

Norepinephrine Transporter 1

Norepinephrine Transporter 1

Adeponectin antibodies

sodium-dependent noradrenaline transporter isoform 2; Sodium-dependent noradrenaline transporter; sodium-dependent noradrenaline transporter; norepinephrine transporter; neurotransmitter transporter; solute carrier family 6 member 5; solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2; Norepinephrine transporter; NET; Solute carrier family 6 member 2

SLC6A2; SLC6A2; NET; NAT1; NET1; SLC6A5; NAT1; NET1; SLC6A5; NET

SC6A2_HUMAN

Polyclonal

Rabbit

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with 0.02% sodium azide. For long-term storage of antibodies, store at -20 degree C. MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in specially

~0.5mg/ml (IgG concentration 0.65-1.25 mg/ml)

Shipped at 4 degree C. Working solutions of antibodies in DilUbuffer should be filtered through 0.45 filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

Antibody is ideal for WB, use of this antibody in other applications has not yet worked out. The dilution of Adpn-101AP antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay. WB; 1:500; Immunoprecipitation & i.p pull-down assays:> n.d

Immunogen: peptide . Reactivity Note: This antibody detects a broad diffuse band of 30 kDa in PC-Adpn smaples. The antibody also reacts to some higher molecular weight proteins that may be multimeric forms of the adiponectin.

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

Obesity related hormone and signal transduction targets

Adepnectin (Adpn) is a protein hormone, exclusively secreted from adipose tissue in to the blood stream that modulates number of metabolic processes, including glucose regulation and fatty acid catabolism. Plasma levels of Adpn are relatively high compared to many other hormones and the concentrations are inversely correlated with body fat percentage in adults, while the association in infants and young children is mofre unclear. Adiponectin is secreted into the bloodsteam where it accounts for approximately 0.01% of all plasma protein at around 5-10 ìg/mL. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Adiponectin exerts some of its weight reduction effects via the brain. This is similar to the action of leptin, but the two hormones perform complementary actions. Adiponectin was first characterized in mice as a transcript overexpressed in preadipocytes (precursors of fat cells) differentiating into adipocytes. The human homologue was identified as the most abundant transcript in adipose tissue. Adiponectin is a 244-amino-acid-long polypeptide, Adpn gene is located on chromosome 3p27a region high lighted as affecting genetic susceptibility to type 2 diabetes. The globular structure of adpn resembles the TNF structure despite unrelated prortein sequences. Primarily the Adpn has three distinct structural motifs, first is a short signal squence that targets the hormone for secretion outside the cell; next is a short variable region thatis species related and the third is a 65-amino acid region with similarity to collagenous proteins; the last is a globular domain. Overall this gene shows similarity to the complement 1Q factors. Adpn exist as self associated homotrimers containing three molecules of Adpn, these homotrimers continue to associate with other Adpn molecules to form hexamers ans dodecamers. The role of these high molecular aggragates remain to be elucidated. Adiponectin binds to a two GPCR that have been identified and characterized. hese have distinct tissue specificities within the body and have different affinities to the various forms of adiponectin. The receptors affect the downstream target AMP kinase, an important cellular metabolic rate control point. Expression of the receptors are correlated with insulin levels, as well as reduced in mouse models of diabetes, particularly in skeletal muscle and adipose tissue. The Adeponectin-selective antibody was generated against a peptide from the globular domain that is unique to Adpn protein. The affinity purified mono epitopespecific rabbit polyclonal antibody strongly labels a 30kDa protein of Adpn in PC-Adpn western blot positive western blot positive control samples. Limited quantities of antigenic blocking peptide for Adpn-101AP and western blot positive controls in ready-to-use buffer are also available. MyBioSource will conjugate Adeponectin antibodies with FITC, Bikotin or other fluorescent probes at a nominal charge.

1. Phornphutkul C, Wu KY, Auyeung V, Chen Q, Gruppuso PA. mTOR signaling contributes to chondrocyte differentiation. Dev Dyn. 2008 Mar;237(3):702-12. 2. Xiangyu Liu and X. F. Steven Zheng. Endoplasmic Reticulum and Golgi Localization Sequences for Mammalian Target of Rapamycin. Mol Biol Cell. 2007 March; 18(3): 1073-1082

4557046

NP_001034.1

NM_001043.3

P23975

69,332 Da

Monoamine Transport Pathway 198851!!Na+/Cl- Dependent Neurotransmitter Transporters Pathway 161070!!SLC-mediated Transmembrane Transport Pathway 119558!!Transmembrane Transport Of Small Molecules Pathway 106572!!Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway 119571

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Function: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Subunit structure: Interacts with PRKCABP. Ref.7. Subcellular location: Membrane; Multi-pass membrane protein. Involvement in disease: Orthostatic intolerance (OI) [MIM:604715]: Syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8. Miscellaneous: This protein is the target of psychomotor stimulants such as amphetamines or cocaine. Sequence similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily. [View classification]

0.1-0.15 ml WB Control

290 USD