antibody

NEDD4 Antibody Affinity Purified

MBS540045

0.1 mg Affinity-Puri

390 USD

polyclonal

rabbit

nonconjugated

mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

NEDD4 Antibody Affinity Purified

NEDD4

Cell proliferation-inducing gene 53 protein; E3 ubiquitin protein ligase Nedd4; KIAA0093; NEDD 4; Neural precursor cell expressed developmentally down regulated 4 antibody

E3 ubiquitin-protein ligase NEDD4; E3 ubiquitin-protein ligase NEDD4; E3 ubiquitin-protein ligase NEDD4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase

NEDD4

Nedd4; Nedd4; Nedd4a; Nedd4a

NEDD4_RAT

Polyclonal

Rabbit

Mouse, Rat

887

Affinity Purified

0.6-0.75 ug/ul in antibody stabilization buffer

-20 degree C for long term storage

ELISA (EIA), IMM, Western Blot (WB)

Dot Blot: 1:10,000. ELISA: 1:10,000. Immunoprecipitation: 1:200. Western Blot: 1:250

Immunogen: Synthetic peptide taken within amino acid region 840-880 on rat Nedd4 protein. Expression: Ubiquitously expressed. Expression is highest in lung, kidney and brain

Molecular Function: Ligase. Subcellular Location: Cell membrane, Cytoplasm, Membrane

Primary Antibodies; Epigenetics; Ubiquitin; NEDD4 Antibody

Affinity Purified E3 ubiquitin-protein ligase NEDD4 Antibody. E3 ubiquitin-protein ligase;Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding

32469607

Q62940.1

Q62940

102,395 Da

Antiviral Mechanism By IFN-stimulated Genes Pathway (1377239); Cytokine Signaling In Immune System Pathway (1377233); Downregulation Of ERBB4 Signaling Pathway (1376955); Endocytosis Pathway (102267); Endocytosis Pathway (102181); Epstein-Barr Virus Infection Pathway (585584); Epstein-Barr Virus Infection Pathway (587115); ISG15 Antiviral Mechanism Pathway (1377240); Immune System Pathway (1377119); Interferon Signaling Pathway (1377234)

may regulate function of the amiloride-sensitive epithelial sodium channel ENaC [RGD, Feb 2006]

NEDD4: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR- 2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Interacts with UBE2D2. Binds SCNN1A, SCNN1B and SCNN1G. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with BEAN1, LITAF, RNF11, WBP1, WBP2, TMEPAI and PRRG2. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes. Interaction with PTEN is questionable according to PubMed:18562292. Interacts with viral proteins that contain a late- budding motif P-P-P-Y. This interaction is essential for viral particle budding of a lot of retroviruses, like HTLV-1 Gag and MLV Gag. Interacts (via C2 domain) with GRB10 (via SH2 domain). Interacts with ERBB4. Interacts with TNIK; the interaction is direct, allows the TNIK-dependent recruitment of RAP2A and its ubiquitination by NEDD4. Interacts (via WW3 domain) with TNK2; EGF promotes this interaction. Interacts (via WW3 domain) with FGFR1 (via C-terminus). Activated by NDFIP1- and NDFIP2-binding. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.3.2.-; Nuclear receptor co-regulator; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase. Cellular Component: cell cortex; chromatin; cytoplasm; cytosol; Golgi apparatus; lipid raft; membrane; microvillus; nucleus; perinuclear region of cytoplasm; plasma membrane; ubiquitin ligase complex. Molecular Function: beta-2 adrenergic receptor binding; ligase activity; phosphoserine binding; phosphothreonine binding; protein binding; protein C-terminus binding; protein domain specific binding; sodium channel inhibitor activity; ubiquitin binding; ubiquitin-protein ligase activity. Biological Process: adaptive immune response; blood vessel morphogenesis; glucocorticoid receptor signaling pathway; immune response; lysosomal transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of vascular endothelial growth factor receptor signaling pathway; neurite development; neuromuscular junction development; positive regulation of nucleocytoplasmic transport; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein catabolic process; progesterone receptor signaling pathway; protein monoubiquitination; protein targeting to lysosome; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; receptor catabolic process; receptor internalization; regulation of dendrite morphogenesis; regulation of membrane potential; regulation of synapse organization and biogenesis; response to denervation involved in regulation of muscle adaptation; T cell activation; transmission of virus; ubiquitin-dependent protein catabolic process via the multivesicular body pathway

0.1 mg Affinity-Purified

390 USD