antibody

Lysyl oxidase Like protein 1 (LOXL1)

MBS540039

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, immunoprecipitation

Antibody

Lysyl oxidase Like protein 1 (LOXL1)

Lysyl oxidase Like protein 1 (LOXL1)

Anti-Lysyl oxidase Like protein 1 (LOXL1); Anti-Lysyl oxidase like 1(LOXL1) Antibody

Loxl1 protein, partial; Lysyl oxidase homolog 1; lysyl oxidase homolog 1; lysyl oxidase 2; lysyl oxidase-like protein 1; lysyl oxidase-like 1; Lysyl oxidase 2; Lysyl oxidase-like protein 1

LOXL1

Loxl1; Loxl1; Loxl; Lox2; Loxl

LOXL1_MOUSE

Polyclonal

Rabbit

Human

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with preservatives. For long-term storage of antibody, store at -20 degree C MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in specially formulat

~0.5mg/ml (Antibodies to LOXL1 was purified on immobilized antigen based affinity chromatography purified immunoglobulin concentration was 0.63-0.66mg/ml in antibody stabilization buffer.)

Shipped at 4 degree C. Working solutions of antibodies in DiluOBuffer should be filtered through 0.45um filter after every use for long-term storage

Western Blot (WB), Immunoprecipitation (IP)

ELISA/dot blot: Antibody dilution 1:20,000-1:50,000;. Western blot: Antibody dilution 1:500-1,000 in diluObuffer. PC-LOXL1 sample 25ul/lane. Other applications (IHC, confocal and Immunoprecipitation for these antibodies are not yet established.

Immunogen: peptide . Reactivity Note: The antibody label a 91kDa and a 32kDa LOXL1 bands in PC-LOXL1 samples

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8. Product Note: 1. Briefly centrifuge to collect liquid, heat or boil PC-LOXL1 tube for 1-2 minutes to dissolve any precipitate before use. This product is "ready-to-use" for electrophoresis. After thawing store at room temperature, Repeated freezing and thawing may result in appearance of higher molecular weight immunoreactive bands. 2. Now Western blots can be stripped and recycle using our specially formulated StripObuffer up to 8 times with out any distortion and significant loss in signal to noise ratios. This stripping buffer does not require heating or have any pungent smell

Antibodies for Developmental biology Research.

Alternate nomenclature/Accession #: Lysyl oxidase like Protein 1 Elastic fibers provide tissues with elasticity which is critical to the function of arteries, lungs, skin, and other dynamic organs. Loss of elasticity is a major contributing factor in aging and diseases. However, the mechanism of elastic fiber development and assembly is poorly understood. Lysyl oxidases are extracellular copper-dependent enzymes that catalyzing the formation of lysine and hydroxylysine-derived cross-links in collagens and lysine-derived cross-links in elastin. These cross-links are essential for the tensile strength of collagens and the rubber-like properties of elastin, both abundant extracellular matrix proteins that are necessary for the structural integrity and function of connective tissues. Lysyl oxidases (LOX) are involved in cross linking of elastin and collagens and the LOX family comprise of LOX and 4 LOX-like enzymes, all are likely to catalyze cross-linked formation in collagens and elastin. The specific functions associated with these isoforms are not completely understood at present. The LOX are essential and crucial for development of the cardiovascular system and LOX null mice died prenatally of aortic aneurysms and cardiovascular dysfunction (1). LOX family members are also essential for respiratory system and the integrity of elastic and collagen fibers in the lungs and skin. Lysyl oxidase catalyzes the reaction that generates peptidyl-ä -hydroxy-á -aminoadipic-ä -semialdehyde and peptidyl-á -aminoadipic-ä -semialdehyde from hydroxylysine and lysine residues which is the final enzymatic reaction required for collagen cross linking. The newly formed aldehyldes fromed by these enzymatic reaction then spontaneously react with other aldehydes, unmodified lysine and hydroxylysine to form a variety fo intra and inter molecular cross links that are found in collagens and elastins (2). Lysyl oxidase is a copper-dependent amine oxidase. It is secreted from fibrogenic cells as a pro-enzyme that appears to have little or no enzyme activity, and is processed in the extracellular environment to produce the active 32 kDa enzyme and an 18 kDa propeptide (3). LOXL1 is a 608aa (605in mouse and 400 in human) protein. Antibodies raised against LOXL1 strongly reacted with a 90kDa protein and a 34 kDa smaller subunit essentially the active LOX subunit in PC-LOXL1 samples. The 400 amino acid sequence in human is processed by cleavage of 207 amino acids form the N-terminal. MyBioSource has made rabbit anti-lysyl oxidase like 1 (LOXL1) antibodies using peptide taken from near the carboxy-terminal end of the protein. The LOXL1- selective antibodies were generated against synthetic peptides form unique regions of the LOXL1 protein. The antigenic peptide was post-synthetically covalently modified to achieve the desired antigenicity before using it as immunogen to immunize rabbits. The rabbit antibodies obtained were isolated and purified on an immobilized antigen based affinity matrix before stabilizing them in antibody stabilization buffer. The antibodies to LOXL1 label LOXL1full length and active subunit as 91kDa and 32 kDa bands in PC-LOXL1 smaples.

1. Joni M. Mäki, Raija Sormunen, et. Al., Am J Pathol. 2005 October; 167(4): 2. Trackman PC, Tang J, Bedell-Hogan D, Kagan HM. J Biol Chem. 1992;267:8666 -8671. 3. Philip C. Trackman., Diverse Biological Functions of Extracellular Collagen Processing Enzymes. J Cell Biochem. 2005 December 1; 96(5): 927-937.

13278295

AAH03973

P97873

66,506 Da

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway 819042!!Collagen Formation Pathway 819040!!Crosslinking Of Collagen Fibrils Pathway 819044!!Elastic Fibre Formation Pathway 819046!!Extracellular Matrix Organization Pathway 819039

Function: Active on elastin and collagen substrates . By similarity. Cofactor: Copper . By similarity.Contains 1 lysine tyrosylquinone . By similarity. Subcellular location: Secreted extracellular space . Potential. Post-translational modification: The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. Sequence similarities: Belongs to the lysyl oxidase family.

0.1-0.15 ml WB Control

290 USD