antibody

Bone morphogenetic protein 4 (BMP 4)

MBS540007

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

BMP

human, mouse, rat

western blot, immunoprecipitation

Antibody

Bone morphogenetic protein 4 (BMP 4)

Bone morphogenetic protein 4 (BMP 4)

Anti-Bone morphogenetic protein 4 (BMP4) antibodies.; Affinity purified BMP2 Antibody

bone morphogenetic protein 4; Bone morphogenetic protein 4; bone morphogenetic protein 4; BMP-4; BMP-2B; bone morphogenetic protein 2B; bone morphogenetic protein 4; Bone morphogenetic protein 2B

BMP 4

Bmp4; Bmp4; BOMPR4A; Bmp-4; Dvr-4; BMP-4; BMP-2B

BMP4_RAT

Polyclonal

Rabbit

Human, mouse, rat

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with preservatives.

~0.5mg/ml (IgG connetration 0.5-0.75 mg/ml)

Shipped at 4 degree C. For long-term storage of antibody, store at -20 degree C MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi-use antibody dilution buffer. Working solutions of antibodies in DiluOBuffer should be filtered through 0.45um filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

ELISA: Antibody dilution 1:2,000 for ELISA or DOT blot assy. W.B: Antibody dilution 1:500-1,000 for WB using PCBMP2. Immunoprecipitation: n.d; IHC n.d

Immunogen: peptide . Reactivity Note: The antibody label 50-52 kDa bone morphogenetic protein 4 in PC-BMP4 sample.

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8. Product Note: Briefly centrifuge to collect liquid, heat or boil PC-BST4 tube for 1-2 minutes to dissolve any precipitate before use. This product is "ready-to-use" for electrophoresis. After thawing store at room temperature, Repeated freezing and thawing may result in appearance of higher molecular weight immunoreactive bands.

Bone Morphogenetic Protein (BMP) Antibodies

The Bone morphogenetic proteins (BMPs) are members of the TGF-beta superfamily of cytokines that are involved in development, differentiation, and disease. T he transforming growth factor-beta (TGF-beta) super-family consists of a large number of growth and differentiation factors, such as TGF-betas, activins, inhibins, growth and differentiation factors (GDFs), and bone morphogenetic proteins (BMPs). These TGF beta ligands act through specific serine/threonine type I and Type II receptor kinases (1). These type I and II receptor kinases subsequently activate Smad proteins, which then propagate the signals into the nucleus to regulate target gene expression. Several BMP isotypes have been identified (BMP1 through BMP16) have been recovered through molecular cloning. Recombinant protein products from several of these clones (BMP2-BMP16) are members of the TGF-beta super-family of regulatory ligands. There exist high degree of amino acid sequence homology between BMP5, BMP6, and BMP7, that constitute a subfamily within the BMPs. The signaling systems of BMP are highly conserved from flies to mammals and have been shown to be important in the development of multiple organs. The formation of skeleton requires a highly regulated inductive signals with antagonist negative feed back system. Inappropriate or excessive expression of BMPs (bone morphogenetic proteins) or their antagonists (Noggin, ectodin) results in genetic disorders affecting the skeleton, such as fibrodysplasia ossificans progressiva. BMP signaling mediated through binding to its receptors is a critical step in the induction of abnormal ossification. BMP-4 expression has been shown in the developmental bone formation in the alveolar bone of rat embryo by immunohistochemistry (2). The bone morphogenetic proteins (BMP) are implicated in several inductive differentiation processes in vertebrate ontogeny and patterning of nervous system. BMP4 and its antagonist Noggin play important role in the CNS development, the expression of BMP4 in rat hippocampus increase gradually and reached its highest levels during weaning and then decline slightly there after (3). BMP4 is also required in normal development of mouse inner ear (3). The BMP4-selective antibodies were generated against peptides form unique regions of the BMP4 protein. MyBioSource has also generated epitope specific rabbit anti-BMP2 and BMP8 polyclonal antibodies utilizing linear and cyclic peptide sequences. These antibodies have been fully characterized for cross reactivity with other members of the bone morphogenetic proteins and other proteins. Limited quantities of the antigenic blocking peptide for BMP2 antibodies is also available.

1. Peng C. J Obstet Gynaecol Can. 2003 Oct;25(10):834-44. 2. Masuhara K, Nakase T, Suzuki S, Takaoka K, Matsui M, Anderson HC. Use of monoclonal antibody to detect bone morphogenetic protein-4 (BMP-4). Bone. 1995 Jan;16(1):91-6. 3. Fan X, Xu H, Cai W, Yang Z, Zhang J. Spatial and temporal patterns of expression of Noggin and BMP4 in embryonic and postnatal rat hippocampus. Brain Res Dev Brain Res. 2003 Dec 19;146(1-2):51-8. 4. Thompson DL, Gerlach-Bank LM, Barald KF, Koenig RJ. Retinoic acid repression of bone morphogenetic protein 4 in inner ear development. Mol Cell Biol. 2003 Apr;23(7):2277-86.

148747216

NP_036959

NM_012827.2

Q06826

46,541 Da

Adipogenesis Pathway 198479!!Basal Cell Carcinoma Pathway 83501!!Basal Cell Carcinoma Pathway 525!!Elastic Fibre Formation Pathway 828438!!Extracellular Matrix Organization Pathway 828431!!Hedgehog Signaling Pathway 83455!!Hedgehog Signaling Pathway 474!!Hippo Signaling Pathway 749790!!Hippo Signaling Pathway 750388!!Molecules Associated With Elastic Fibres Pathway 828439

plays a role in induction of cell proliferation [RGD, Feb 2006]

Function: Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction . By similarity. Subunit structure: Homodimer; disulfide-linked. Interacts with SOSTDC1, GREM2, RGMA, RGMB and RGMC. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition . By similarity. Subcellular location: Secreted extracellular space extracellular matrix. Sequence similarities: Belongs to the TGF-beta family.

0.1-0.15 ml WB Control

290 USD