antibody

Aquarious AQR

MBS540004

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

Antibody

Aquarious AQR

Aquarious AQR

Anti-Aquarius antibodies

intron-binding protein aquarius; Intron-binding protein aquarius; intron-binding protein aquarius; intron-binding protein of 160 kDa; functional spliceosome-associated protein 164; aquarius homolog (mouse); Intron-binding protein of 160 kDa

AQR

AQR; AQR; IBP160; fSAP164; KIAA0560; IBP160

AQR_HUMAN

Polyclonal

Rabbit

Affinity purifed

The antiserum is supplied in antibody stabilization buffer.

~0.5mg/ml (IgG concentration 1-1.25 mg/ml in antibody stabilization buffer)

Shipped at 4 degree C. Store at -20 degree C for long-term storage. MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer (DiluOBuffer, Cat# FGI 1963). Working solutions of antibodies in DiluOBuffer should be filtered through 0.45um filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

Recommended dilutions: WB 1:500; Immunoprecipitation and & i.p pull-down assays: n.d. Antibodies is used for W B applications at 1:500 dilutions. Other applications for this antibody have not been tested. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay in his/her laboratory. AQR-101 applications in Immunoprecipitation and IHC are not yet standardized.

Immunogen: Peptide (14mer). Epitope Location: 300-340. Reactivity Note: Detects an approximately 140 kDa AQR protein in AQR western blot positive control sample and in several mammalian breast cancer cells

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

Antibodies to Cell Differentiation marker proteins

The gene trap screen methodology was used to identify and characterize a new and novel gene from retinoic acid dependent morphologically differentiated mouse embryonic stem cells, called Aquarious (AQR). The promoterless lacZ marker was used to trap the genomic locus and to determine the expression pattern of the gene. AQR transcripts are strongly induced in response to RA in vitro in mouse embryonic stem cells (1). The expression of AQR during embryogenesis was localized in the mesoderm and neuronal crest region and its target tissue and in neuroepithelium. Expression was first detected at 8.5 days postcoitum, when neural crest cells are visible at the lateral ridges of the neural plate. The gene-trapped AQR locus was transmitted through the mouse germ line in three genetic backgrounds. The AQR gene ratio in the F2 generation was 1:2:1 as predicted by the mendalion distribution and was observed in all the backgrounds suggesting that homozygous mice are viable. The homozygous mice are normal in weight and size and breed normally. The gene trap insertion, however, does not seem to generate a null mutation, because AQR transcripts are still present in the homozygous mutant animals. The AQR open reading frame has weak homology to RNA-dependent RNA polymerases (RRPs) of the murine hepatitis viruses and contains an RRP motif. AQR was mapped to mouse chromosome 2 between regions E5 through F2 by using fluorescence in situ hybridization analysis. (1). The AQR-selective antibodies were generated against synthetic peptide corresponding to residues 273-291 of mouse embryonic stem cell AQR kinase. The antibodies to AQR are affinity purified over immobilized antigen matrix based chromatography. The purified antibodies are stabilized in antibody stabilization buffer containing preservatives. MyBioSource will also provide Western blot positive controls for Aquorious protein in ready-to-use buffer for SDS-PAGE and Western blot analyses. These positive controls can be used as internal standards for quantitaive western blotting purposes. MyBioSource. also carries limited quantities of antigenic blocking peptide for AQR antibodies. Please inquire about pricing and availability of these products. Other related antibodies that are carried by MyBioSource include vitamin A transporter Strah6, p21 activated protein kinase (p65pak), ACK1, Anti-protein synthesis initiation factor 4 (eIF4E), Anti-protein phosphatase 2A inhibitor protein.

1. Sam M, Wurst W, Kluppel M, Jin O, Heng H, Bernstein A. Aquarius, a novel gene isolated by gene trapping with an RNA-dependent RNA polymerase motif. Dev Dyn. 1998 Jun;212(2):304-17

38788372

NP_055506

NM_014691.2

O60306

171,295 Da

Spliceosome Pathway 125136!!Spliceosome Pathway 124832!!Spliceosome, 35S U5-snRNP Pathway 413438

Function: Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Ref.5. Subunit structure: Identified in the spliceosome C complex. Ref.4. Subcellular location: Nucleus. Note: Localizes to speckle-like regions of the nucleoplasm. Ref.5. Sequence similarities: Belongs to the CWF11 family. Sequence caution: The sequence BAA25486.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.1-0.15 ml WB Control

290 USD