antibody

Adiponectin 1

MBS540002

0.1-0.15 ml WB Contr

290 USD

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

Antibody

Adiponectin 1

Adiponectin 1

Adeponectin antibodies; Affinity purified rabbit Adeponectin Antibody

Sus scrofa adiponectin mRNA, complete cds; adiponectin

ADIPOQ; ADN; APM1; ACRP30

Polyclonal

Rabbit

Human, mouse, rat, pig

Affinity purifed

The antiserum is supplied in antibody stabilization buffer with 0.02% sodium azide.

~0.5mg/ml

Shipped at 4 degree C. For long-term storage of antibodies, store at -20 degree C. MyBioSource does not recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer. Working solutions of antibodies in DilUbuffer should be filtered through 0.45 filter after every use for long-term storage.

Western Blot (WB), Immunoprecipitation (IP)

WB: 1:500; Immunoprecipitation & i.p pull-down assays:> n.d. Antibody is ideal for WB, use of this antibody in other applications has not yet worked out. The dilution of Adpn-101AP antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay

Immunogen: peptide (19mer). Epitope Location: 190-225. Reactivity Note: This antibody detects a broad diffuse band of 30 kDa in PC-Adpn smaples. The antibody also reacts to some higher molecular weight proteins that may be multimeric forms of the adiponectin

Preservatives: 0.02% Na3N. Buffer: PO4-buffer/Tris/Glycine, pH 7.4-7.8. PO4-buffer/Tris/Glycine, pH 7.4-7.8

Obesity related hormone and signal transduction targets

Alternate nomenclature: Acrp30 and apM1 protein Adepnectin (Adpn) is a protein hormone, exclusively secreted from adipose tissue in to the blood stream that modulates number of metabolic processes, including glucose regulation and fatty acid catabolism. Plasma levels of Adpn are relatively high compared to many other hormones and the concentrations are inversely correlated with body fat percentage in adults, while the association in infants and young children is mofre unclear. Adiponectin is secreted into the bloodsteam where it accounts for approximately 0.01% of all plasma protein at around 5-10 ug/mL. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Adiponectin exerts some of its weight reduction effects via the brain. This is similar to the action of leptin, but the two hormones perform complementary actions. Adiponectin was first characterized in mice as a transcript overexpressed in preadipocytes (precursors of fat cells) differentiating into adipocytes. The human homologue was identified as the most abundant transcript in adipose tissue. Adiponectin is a 244-amino-acid-long polypeptide, Adpn gene is located on chromosome 3p27a region high lighted as affecting genetic susceptibility to type 2 diabetes. The globular structure of adpn resembles the TNF structure despite unrelated prortein sequences. Primarily the Adpn has three distinct structural motifs, first is a short signal squence that targets the hormone for secretion outside the cell; next is a short variable region thatis species related and the third is a 65-amino acid region with similarity to collagenous proteins; the last is a globular domain. Overall this gene shows similarity to the complement 1Q factors. Adpn exist as self associated homotrimers containing three molecules of Adpn, these homotrimers continue to associate with other Adpn molecules to form hexamers ans dodecamers. The role of these high molecular aggragates remain to be elucidated. Adiponectin binds to a two GPCR that have been identified and characterized. hese have distinct tissue specificities within the body and have different affinities to the various forms of adiponectin. The receptors affect the downstream target AMP kinase, an important cellular metabolic rate control point. Expression of the receptors are correlated with insulin levels, as well as reduced in mouse models of diabetes, particularly in skeletal muscle and adipose tissue. The Adeponectin-selective antibody was generated against a peptide from the globular domain that is unique to Adpn protein. The affinity purified mono epitopespecific rabbit polyclonal antibody strongly labels a 30kDa protein of Adpn in PC-Adpn western blot positive western blot positive control samples. Limited quantities of antigenic blocking peptide for Adpn-101AP and western blot positive controls in ready-to-use buffer are also available. MyBioSource will conjugate Adeponectin antibodies with FITC, Bikotin or other fluorescent probes at a nominal charge. MyBioSource also provides antibodies against many GPCRs, leptin and other obesity related proteins

1. Phornphutkul C, Wu KY, Auyeung V, Chen Q, Gruppuso PA. mTOR signaling contributes to chondrocyte differentiation. Dev Dyn. 2008 Mar;237(3):702-12. 2. Xiangyu Liu and X. F. Steven Zheng. Endoplasmic Reticulum and Golgi Localization Sequences for Mammalian Target of Rapamycin. Mol Biol Cell. 2007 March; 18(3): 1073-1082

46520142

AY589691

Adipocytokine Signaling Pathway 84504!!Adipocytokine Signaling Pathway 505!!PPAR Signaling Pathway 84457!!PPAR Signaling Pathway 450!!Type II Diabetes Mellitus Pathway 84505!!Type II Diabetes Mellitus Pathway 506

0.1-0.15 ml WB Control

290 USD