product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
AAV5 ELISA Kit
catalog :
MBS539704
quantity :
96 Tests
price :
1260 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS539704
products type :
ELISA Kit
products full name :
AAV5 ELISA Kit
products short name :
[AAV5]
products name syn :
[AAV-5 ELISA Kit; Adeno Associated Virus 5 ELISA Kit; AAV5 Titration ELISA Kit]
products gene name :
[AAV5]
storage stability :
Store at 4 degree C.
tested application :
ELISA (EIA)
image1 heading :
Typical Testing Data/Standard Curve
other info1 :
Assay Type: Sandwich
products categories :
Infectious Disease
products description :
Principle of the Assay The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV 5 capsids is coated onto microtiter strips and is used to capture AAV 5 particles from the specimen. Captured AAV 5 particles are detected in two steps. First a biotin conjugated monoclonal antibody to AAV 5 is bound to the immune complex. In the second step streptavidin peroxidase conjugate reacts with the biotin molecules. Addition of substrate solution results in a color reaction which is proportional to the amount of specifically bound viral particles. The absorbance is measured photometrically at 450 nm. The kit control provided contains an AAV 5 particle preparation of intact capsids. It shows a typical standard curve when used in serial dilution. It allows quantitative determination of samples of an unknown particle titre (immunological titre) and the calibration of an inhouse AAV 5 preparation (e.g. infectious titre, transducing units, DNA dot blot titre). Background/Introduction: Adeno-associated virus 5 (AAV 5) is a ssDNA virus that is a topic of intense study in gene therapy especially for the treatment of cystic fibrosis where it shows long term expression. AAV 5 was isolated from a human genital site and has been used in several studies showing superior infection of lung, central nervous system, muscle, eye and arthritic joints when compared to other AAV serotypes. AAV 5 structure has been analyzed by X-ray crystallographic studies. Methods for the characterisation of AAV preparations currently include titration ELISA, real-time PCR, DNA dot blot, determination of transducing units, infectious center assay, SDS-PAGE and electron microscopy.
size1 :
96 Tests
price1 :
1260 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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