Klotho protein | MyBioSource MBS539406 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Klotho protein

catalog :

MBS539406

quantity :

0.02 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS539406

products type :

Recombinant Protein

products full name :

Klotho protein

products short name :

Klotho

products name syn :

aKlotho protein; KLA protein

other names :

klotho; Klotho; klotho; klotho

other gene names :

KL; KL

uniprot entry name :

KLOT_HUMAN

host :

Human

sequence length :

1012

sequence :

Region of Klotho protein corresponding to amino acids EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH.

purity :

> 98% pure

form :

Supplied as a lyophilized powder

concentration :

20 ug/vial; lyophilized

storage stability :

Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

tested application :

User optimized

other info1 :

Protein Type: Recombinant. Biological Significance: Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon,. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid. Bioactivity: Determined by the dose-dependent stimulation of the proliferation of Mouse NIH-3T3 cells. Recombinant human Klotho is effective in a concentration range of 0.5-2.0 ug/ml. Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

other info2 :

Expression System: CHO cells

products categories :

Nutrition & Metabolism

products description :

Purified recombinant Human Klotho protein

ncbi gi num :

2618596

ncbi acc num :

BAA23382.1

uniprot acc num :

Q9UEF7

ncbi mol weight :

62,135 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Constitutive Signaling By Aberrant PI3K In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Diseases Of Signal Transduction Pathway (1183518); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR1 Pathway (1183557); Downstream Signaling Of Activated FGFR2 Pathway (1183564)

ncbi summary :

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]

uniprot summary :

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; EC 3.2.1.31; Membrane protein, integral. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: extracellular space; integral to plasma membrane; extracellular region; integral to membrane; plasma membrane. Molecular Function: vitamin D binding; signal transducer activity; beta-glucosidase activity; beta-glucuronidase activity; fibroblast growth factor binding; hormone activity; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; calcium ion homeostasis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging. Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial

size1 :

0.02 mg

price1 :

310 USD

more info or order :

MyBioSource product webpage