protein

sFRP1 protein

MBS539401

0.02 mg

310 USD

Recombinant Protein

sFRP1 protein

sFRP1

SFRP-1 protein; Secreted Frizzled Related Protein 1 protein; sFRP-1 protein; sFRP 1; sFRP 1 protein; Secreted Apoptosis Related Protein-2 protein; sFRP-1; sFRP1; Soluble Frizzled Related Protein-1 protein; SARP-2 protein

Secreted frizzled-related protein 1; Secreted frizzled-related protein 1; secreted frizzled-related protein 1; secreted frizzled-related protein 1; Secreted apoptosis-related protein 2; SARP-2

sFRP1

SFRP1; SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2; FRP; FRP1; SARP2; FRP-1; sFRP-1; SARP-2

SFRP1_HUMAN

Human

314

SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK

> 97% pure

Supplied as a lyophilized powder

Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

User optimized

Protein Type: Recombinant. Biological Significance: Secreted Frizzled Related Proteins (sFRPs) modulate WNT signaling by binding directly to WNT proteins in a manner that affects their receptor binding and signaling capabilities. sFRP-1 is a widely distributed protein that can bind directly to WNT1, WNT2, and possibly other WNT proteins, and generally exerts anti-proliferative effects consistent with activity as a WNT antagonist. It also inhibits apoptosis and has been found to be down-regulated in many solid tumors, but up-regulated in uterine leiomyomas. Bioactivity: Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells. The expected ED50 is 0.3-0.5 ug/ml. Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

Expression System: HeLa cells

Cancer

Purified recombinant Human sFRP1 protein

22209051

AAH36503.1

Q8N474

35,386 Da

Signal Transduction Pathway (477114); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (1127516); Validated Targets Of C-MYC Transcriptional Repression Pathway (169353); Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471); Negative Regulation Of TCF-dependent Signaling By WNT Ligand Antagonists Pathway (1127525)

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p11.21. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; extracellular region; plasma membrane; integral to membrane; intracellular; cytosol. Molecular Function: heparin binding; G-protein coupled receptor activity; Wnt-protein binding; identical protein binding; protein binding; Wnt receptor activity; frizzled binding; cysteine-type endopeptidase activity; drug binding. Biological Process: negative regulation of osteoclast differentiation; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; proteolysis; response to organic cyclic substance; negative regulation of BMP signaling pathway; negative regulation of insulin secretion; negative regulation of cell proliferation; positive regulation of focal adhesion formation; ureteric bud development; positive regulation of stress fiber formation; positive regulation of cell proliferation; negative regulation of ossification; positive regulation of smoothened signaling pathway; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; positive regulation of Wnt receptor signaling pathway; negative regulation of cell migration; female gonad development; negative regulation of epithelial cell proliferation; response to drug; negative regulation of B cell differentiation; negative regulation of JNK activity; negative regulation of peptidyl-tyrosine phosphorylation; menstrual cycle phase; male gonad development; digestive tract morphogenesis; cellular response to starvation; positive regulation of cell growth; regulation of angiogenesis; osteoblast differentiation; negative regulation of fibroblast proliferation; negative regulation of bone remodeling; neural tube closure; positive regulation of fat cell differentiation; neural crest cell fate commitment; hemopoietic progenitor cell differentiation; negative regulation of osteoblast proliferation; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

0.02 mg

310 USD