catalog number :
MBS539396
products type :
Recombinant Protein
products full name :
PAFAH protein
products short name :
PAFAH
products name syn :
PAF 2-acylhydrolase protein; PAF acetylhydrolase protein; LDL-associated phospholipase A2 protein; LDL-PLA2 protein; 2-acetyl-1-alkylglycerophosphocholine esterase protein; 1-alkyl-2-acetylglycerophosphocholine esterase protein
other names :
platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase
products gene name :
PAFAH
other gene names :
PLA2G7; PAF-AH; Lp-PLA2
sequence :
Region of PAFAH protein corresponding to amino acids FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN.
form :
Supplied as a lyophilized powder
storage stability :
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
tested application :
User optimized
other info1 :
Protein Type: Recombinant. Biological Significance: Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the ?sn2? ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. Bioactivity: Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 ug/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes. Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
other info2 :
Expression System: 293 cells
products categories :
Immunology
products description :
Purified recombinant Human PAFAH protein
ncbi acc num :
NP_001106484.1
ncbi gb acc num :
NM_001113013.1
ncbi mol weight :
47-55 kDa
ncbi pathways :
Biosynthesis Of Antibiotics Pathway 1146434!!Ether Lipid Metabolism Pathway 84416!!Ether Lipid Metabolism Pathway 365!!Metabolic Pathways 132936!!Metabolism Of Proteins Pathway 1181766!!Peptide Hormone Metabolism Pathway 1181820!!Synthesis, Secretion, And Deacylation Of Ghrelin Pathway 1181822