protein

SLIT2N protein

MBS539387

0.025 mg

310 USD

Recombinant Protein

SLIT2N protein

SLIT2N

SLIL3 protein; Slit homolog 2 protein protein; Slit-2 N protein

SLIT2N

HEK293 cells

Region of SLIT2 N protein corresponding to amino acids QACPAQCSCS GSTVDCHGLA LRSVPRNIPR NTERLDLNGN NITRITKTDF AGLRHLRVLQ LMENKISTIE RGAFQDLKEL ERLRLNRNHL QLFPELLFLG TAKLYRLDLS ENQIQAIPRK AFRGAVDIKN LQLDYNQISC IEDGAFRALR DLEVLTLNNN NITRLSVASF NHMPKLRTFR LHSNNLYCDC HLAWLSDWLR QRPRVGLYTQ CMGPSHLRGH NVAEVQKREF VCSGHQSFMA PSCSVLHCPA ACTCSNNIVD CRGKGLTEIP TNLPETITEI RLEQNTIKVI PPGAFSPYKK LRRIDLSNNQ ISELAPDAFQ GLRSLNSLVL YGNKITELPK SLFEGLFSLQ LLLLNANKIN CLRVDAFQDL HNLNLLSLYD NKLQTIAKGT FSPLRAIQTM HLAQNPFICD CHLKWLADYL HTNPIETSGA RCTSPRRLAN KRIGQIKSKK FRCSAKEQYF IPGTEDYRSK LSGDCFADLA CPEKCRCEGT TVDCSNQKLN KIPEHIPQYT AELRLNNNEF TVLEATGIFK KLPQLRKINF SNNKITDIEE GAFEGASGVN EILLTSNRLE NVQHKMFKGL ESLKTLMLRS NRITCVGNDS FIGLSSVRLL SLYDNQITTV APGAFDTLHS LSTLNLLANP FNCNCYLAWL GEWLRKKRIV TGNPRCQKPY FLKEIPIQDV AIQDFTCDDG NDDNSCSPLS RCPTECTCLD TVVRCSNKGL KVLPKGIPRD VTELYLDGNQ FTLVPKELSN YKHLTLIDLS NNRISTLSNQ SFSNMTQLLT LILSYNRLRC IPPRTFDGLK SLRLLSLHGN DISVVPEGAF NDLSALSHLA IGANPLYCDC NMQWLSDWVK SEYKEPGIAR CAGPGEMADK LLLTTPSKKF TCQGPVDVNI LAKCNPCLSN PCKNDGTCNS DPVDFYRCTC PYGFKGQDCD VPIHACISNP CKHGGTCHLK EGEEDGFWCI CADGFEGENC EVNVDDCEDN DCENNSTCVD GINNYTCLCP PEYTGELCEE KLDFCAQDLN PCQHDSKCIL TPKGFKCDCT PGYVGEHCDI DFDDCQDNKC KNGAHCTDAV NGYTCICPEG YSGLFCEFSP PMV.

> 98% pure by SDS-PAGE

Sterile filtered and lyophilized from 20mM Tris and 200mM L-Arginine, pH 8.8. Centrifuge vial prior to opening. Reconsitute in 20mM Tris, pH 8.8 with 150mM NaCl for a concentration of 0.1 to 1.0mg/ml. Do not Vortex. Note: slow to dissolve

25 ug/vial; Lyophilized

Store at -20 degree C. The undiluted solution can be stored at 4 degree C for up to 1 week. Working aliquots sotered with a carrier protein are stable for at alead 3 months at -20 degreee C to -80 degree C. Avoid freeze/thaw cycles.

User optimized

Protein Type: Recombinant. Biological Significance: SLIT2is a member of the Slit family that signals through the Roundabout (Robo) receptor as a repellent for axon guidance and neuronal migration, and can also act as a chemoattractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. SLIT2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in adult adrenal gland, thyroid and trachea. SLIT2 is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and SLIT2-C respectively. The neurodevelopment related activities, as measured by the ability to repel olfactory bulb axons and to induce branching in dorsal root ganglia axons, are contained only in the N-terminal fragment. Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug)

Expression System: 293 cells. Biohazard: Use normal laboratory precautions when handling reagent.

Neuroscience

Purified recombinant Human SLIT2N protein

120-140 kDa

0.025 mg

310 USD