protein

DKK1 protein

MBS539371

0.01 mg

310 USD

Recombinant Protein

DKK1 protein

DKK1

Dickkopf-related protein-1 protein; DKK 1; Dickkopt-1 protein; SK protein; DKK-1 protein; DKK-1; DKK 1 protein; DKK1

dickkopf-related protein 1; Dickkopf-related protein 1; dickkopf-related protein 1; dickkopf WNT signaling pathway inhibitor 1; SK

DKK1

DKK1; DKK1; SK; DKK-1; Dickkopf-1; Dkk-1; hDkk-1

DKK1_HUMAN

Human

266

Region of DKK1 protein corresponding to amino acids TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH.

> 97% pure

Lyophilized from PBS buffer, pH 7.4

Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

User optimized

Protein Type: Recombinant. Biological Significance: DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/betacatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/betacatenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Bioactivity: Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200ng/ml. Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

Expression System: 293 cells

Differentiation & Development

Human recombinant DKK1 protein

7110719

NP_036374.1

NM_012242.2

O94907

35-40 kDa

Cardiac Progenitor Differentiation Pathway (712094); Direct P53 Effectors Pathway (137939); Disease Pathway (530764); Diseases Of Signal Transduction Pathway (1183518); Presenilin Action In Notch And Wnt Signaling Pathway (137914); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1127614); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (1127516)

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family. Protein type: Secreted, signal peptide; Secreted; Inhibitor. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: extracellular space; early endosome membrane; plasma membrane; extracellular region. Molecular Function: receptor antagonist activity; signal transducer activity; protein binding; growth factor activity; low-density lipoprotein receptor binding. Biological Process: negative regulation of skeletal muscle development; cellular morphogenesis during differentiation; negative regulation of Wnt receptor signaling pathway; response to retinoic acid; regulation of receptor internalization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; mesoderm formation; negative regulation of mesodermal cell fate specification; hair follicle development; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis

0.01 mg

310 USD