catalog number :
MBS539294
products type :
Substrate
products full name :
TMB ELISA Substrate
products short name :
[TMB]
products gene name :
[TMB]
form :
Liquid ultra sensitive substate for ELISA mircowell applications containing TMB (3,3,5,5-tetramethylbenzidine) in a mildly acidic buffer. Mixed substrate/chromogen. Excellent for microwell peroxidase immunoassay systems
concentration :
Ready to use @ 100ul per well. Use this product at room temperature
storage stability :
Store at 4 degree C. Product is light sensitive: protect from direct sunlight and UV sources.
tested application :
ELISA (EIA)
app notes :
Optimal conditions to be determined by end user.
other info1 :
Biological Significance: This HRP Microwell Substrate contains 3,3?,5,5?-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution. Unreacted substrate should be colorless or very light yellow in appearance. When this substrate system is reacted with peroxidase, a soluble blue reaction product is obtained. The reaction can be stopped using appropriate stop solution, producing a soluble yellow or soluble blue reaction product, depending upon the stop reagent used, which is stable for at least 1 hour. Biohazard: Non-hazardous, mild oxidizing agent, no organic solvents. Use laboratory practices when handling; in case of contact flush with water. Note: In kinetic assays. TMB develops a deep blue color in the presence of peroxidase and can be read at 370 or 650nm. In endpoint assays, TMB develops a yellow color and can be read at 450nm approximately 30 to 60 minutes after adding stopping reagent.
products categories :
Biological Reagents; Substrates
products description :
High sensitivity HRP microwell substrate (containing TMB)