catalog number :
MBS539178
products type :
Secondary Antibody
products full name :
Donkey anti Sheep IgG (H + L) (HRP)
products short name :
[Donkey anti Sheep IgG (H + L) (HRP)]
products name syn :
[Donkey anti Sheep Immunoglobulin G (H + L) secondary (HRP); Dnk x Sp IgG (H + L)(HRP)]
products gene name :
[IgG]
purity :
Donkey anti Sheep IgG (H + L) (HRP) was purified by affinity chromatography.
form :
Supplied lyophilized from 10mM Sodium Phosphate, 250mM Sodium Chloride, pH 7.6, with 15mg/ml BSA. Reconstitute with 0.5 ml of distilled Water.
concentration :
0.5ml per vial; lyophilzed; antibody concentration is 0.8mg/ml
storage stability :
Ships ambient or refrigerated. Upon receipt store at 4 degree C until reconstituted. After reconstitution aliquot and store at -70 degree C or diluted with an equal volume of Glycerol and stored at -20 degree C.
tested application :
ELISA (EIA),Immunohistochemistry (IHC), Western Blot (WB)
app notes :
Immunohistochemistry: 1:500 – 1:5,000. ELISA and Western Blotting with chromogenic substrates: 1:5,000–1:100,000. Western Blotting with ECL substrates: 1:10,000-1:200,000
other info1 :
Biological Significance: Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells.
other info2 :
Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling. Tag/Conjugate: HRP. Immunogen: Antisera purified by immunoaffinity chromatography using antigens coupled to agarose beads. Cross reactivity: Ab reacts with whole molecule Sheep IgG and with light chains common to most sheep immunoglobulins. Minimally cross reactive with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit and rat serum proteins. Warning: BSA and Dry Milk may contain IgG which reacts with anti-sheep IgG.
products categories :
Immunology; Conjugated Secondary Antibodies
products description :
Donkey anti Sheep IgG (H + L) secondary antibody (HRP)