protein

WISP1 protein

MBS538315

0.02 mg

315 USD

Recombinant Protein

WISP1 protein

WISP1

WNT-1 inducible signaling pathway protein-1 protein; CCN4 protein; WISP-1 protein; WISP-1; WISP1; WISP 1 protein; Wnt-1-induced secreted protein protein; WISP 1

WNT1-inducible-signaling pathway protein 1 isoform 2; WNT1-inducible-signaling pathway protein 1; WNT1-inducible-signaling pathway protein 1; WNT1 inducible signaling pathway protein 1; CCN family member 4; Wnt-1-induced secreted protein

WISP1

WISP1; WISP1; CCN4; WISP1c; WISP1i; WISP1tc; CCN4; WISP-1

WISP1_HUMAN

Human

280

Region of WISP1 protein corresponding to amino acids TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN.

> 98% pure

Lyophilized from 10 mM CH3COOH. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml (this product is slow to dissolve)

Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

User optimized

Protein Type: Recombinant. Biological Significance: WISP-1 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is expressed in the heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. WISP-1 is a beta catenin regulated protein that can contribute to tumorigenesis and has also been shown to play a role in bone development and fracture repair. Human WISP-1 is a 38.1 kDa protein containing 346 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cystine knot-like (CTCK) domain. Bioactivity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 ug/ml. Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

Expression System: E Coli

Cytokines & Growth Factors

Purified recombinant Human WISP1 protein

18491000

NP_543028.1

NM_080838.2

O95388

21,550 Da

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]

WISP1: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Belongs to the CCN family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Oncoprotein; Secreted. Chromosomal Location of Human Ortholog: 8q24.22. Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm. Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding. Biological Process: Wnt receptor signaling pathway; cell-cell signaling; regulation of cell growth; cell adhesion; signal transduction

0.02 mg

315 USD