catalog number :
MBS538123
products type :
Recombinant Protein
products full name :
Wnt1 protein
products short name :
Wnt1
products name syn :
Wnt 1 protein; Wnt-1 protein; Wnt1; Wnt 1; Wnt-1; INT-1 protein
other names :
Wnt1, partial; Protein Wnt
products gene name :
Wnt1
uniprot entry name :
C4P689_OCTBM
sequence :
Region of Wnt1 protein corresponding to amino acids ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL.
form :
Supplied lyophilized with no additives. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml.
storage stability :
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
tested application :
User optimized
other info1 :
Protein Type: Recombinant. Biological Significance: Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Bioactivity: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by Mouse ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2. Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
other info2 :
Expression System: E Coli
products categories :
Cytokines & Growth Factors
products description :
Purified recombinant Human Wnt1 protein
ncbi acc num :
ACR19850.1
ncbi mol weight :
38.4 kDa