MyBioSource

protein

PAP protein (> 95% pure)

MBS537823

0.1 mg

270 USD

MBS537823

Native Protein

PAP protein (> 95% pure)

PAP (> 95% pure)

PAP protein; Prostatic Acid Phosphatase protein; PAP antigen

prostatic acid phosphatase isoform PAP; Prostatic acid phosphatase; prostatic acid phosphatase; TMPase; 5'-nucleotidase; ecto-5'-nucleotidase; thiamine monophosphatase; prostatic acid phosphotase; acid phosphatase, prostate; 5'-nucleotidase (EC:3.1.3.5); 5'-NT; Ecto-5'-nucleotidase; Thiamine monophosphatase; TMPase

PAP

ACPP; ACPP; PAP; ACP3; 5'-NT; ACP-3; PAP; 5'-NT; TMPase

PPAP_HUMAN

Human seminal fluid

Human

Highly pure grade; >95% pure by SDS-PAGE

Supplied as a liquid in 150mM NaCl, 9.5mM Sodium Phosphate Monobasic, 40.5mM Sodium Phosphate Dibasic, with 0.05% Sodium Azide as a preservative, pH 7.6, 0.2 um sterile filtered.

3.84 mg/ml

Aliquot and store at -20 DEG C. Avoid repeated Freeze/Thaw cycles.

Biohazard Information: Donor samples were tested and found to be negative for  HIV I/II, and HCV antibodies, and Hepatitis B surface antigen. Nonetheless caution should be used when handling this material as there is a margin of error in all tests. This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Specific Activity: 244.65 U/mg

Cancer; Proteins; Native & Recombinant Proteins; Native

Purified native Human PAP protein (> 95% pure) . Prostatic acid phosphatase (PAP), also prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases. The highest levels of acid phosphatase are found in metastasized prostate cancer.

6382064

NP_001090.2

NM_001099.4

P15309

51 kDa

Riboflavin Metabolism Pathway 83012!!Riboflavin Metabolism Pathway 398

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Function: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Ref.11 Ref.13 Ref.15 Ref.18Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. Ref.11 Ref.13 Ref.15 Ref.18. Catalytic activity: A phosphate monoester + H2O = an alcohol + phosphate. Ref.11A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. Ref.11. Enzyme regulation: Phosphatase activity inhibited by L+-tartrate, and by its derivative, alpha-benzylaminobenzylphosphonic acid. Ref.10. Subunit structure: Homodimer; dimer formation is required for phosphatase activity . By similarity. Subcellular location: Isoform 1: Secreted Ref.12 Ref.14. Isoform 2: Cell membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: Appears to shuttle between the cell membrane and intracellular vesicles. Colocalizes with FLOT1 at cell membrane and in intracellular vesicles. Colocalizes with LAMP2 on the lysosome membrane. Ref.12 Ref.14. Tissue specificity: Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Isoform 2 also expressed in the sarcolemma of skeletal muscle. Levels of this cellular isoform decreased in prostate cancer. Ref.12 Ref.19. Post-translational modification: N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Ref.9 Ref.21 Ref.22Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection. Ref.13 Ref.15. Miscellaneous: Used as a diagnostic tool for staging metastatic prostatic cancer. Sequence similarities: Belongs to the histidine acid phosphatase family.

0.1 mg

270 USD