PAP protein (> 40% pure) | MyBioSource MBS537789 product information

product summary

request information :

company name :

MyBioSource

product type :

protein

product name :

PAP protein (> 40% pure)

catalog :

MBS537789

quantity :

1 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS537789

products type :

Native Protein

products full name :

PAP protein (> 40% pure)

products short name :

PAP (> 40% pure)

products name syn :

PAP protein; PAP antigen; Prostatic Acid Phosphatase protein

other names :

prostatic acid phosphatase isoform PAP; Prostatic acid phosphatase; prostatic acid phosphatase; TMPase; 5'-nucleotidase; ecto-5'-nucleotidase; thiamine monophosphatase; prostatic acid phosphotase; acid phosphatase, prostate; 5'-nucleotidase (EC:3.1.3.5); 5'-NT; Ecto-5'-nucleotidase; Thiamine monophosphatase; TMPase

products gene name :

PAP

other gene names :

ACPP; ACPP; PAP; ACP3; 5'-NT; ACP-3; PAP; 5'-NT; TMPase

uniprot entry name :

PPAP_HUMAN

host :

Human seminal fluid

reactivity :

Human

purity :

Partial purity grade; >40% pure

form :

Supplied 0.2 micron filtered in 150mM NaCl, 9.5mM Sodium Phosphate Monobasic, 40.5mM Sodium Phosphate Dibasic, pH 7.6 with 0.05% NaN3

storage stability :

Aliquot and store at -20 DEG C. Avoid repeated Freeze/Thaw cycles.

other info2 :

Biohazard Information: Donor samples were tested and found to be negative for HIV I/II, and HCV antibodies, and Hepatitis B surface antigen. Nonetheless caution should be used when handling this material as there is a margin of error in all tests. This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

products categories :

Cancer; Proteins; Native & Recombinant Proteins; Native

products description :

Purified native Human PAP protein (> 40% pure) . Prostatic acid phosphatase (PAP), also prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases. The highest levels of acid phosphatase are found in metastasized prostate cancer.

ncbi gi num :

6382064

ncbi acc num :

NP_001090.2

ncbi gb acc num :

NM_001099.4

uniprot acc num :

P15309

ncbi mol weight :

51 kDa

ncbi pathways :

Riboflavin Metabolism Pathway 83012!!Riboflavin Metabolism Pathway 398

ncbi summary :

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

uniprot summary :

Function: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Ref.11 Ref.13 Ref.15 Ref.18Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. Ref.11 Ref.13 Ref.15 Ref.18. Catalytic activity: A phosphate monoester + H2O = an alcohol + phosphate. Ref.11A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. Ref.11. Enzyme regulation: Phosphatase activity inhibited by L+-tartrate, and by its derivative, alpha-benzylaminobenzylphosphonic acid. Ref.10. Subunit structure: Homodimer; dimer formation is required for phosphatase activity . By similarity. Subcellular location: Isoform 1: Secreted Ref.12 Ref.14. Isoform 2: Cell membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: Appears to shuttle between the cell membrane and intracellular vesicles. Colocalizes with FLOT1 at cell membrane and in intracellular vesicles. Colocalizes with LAMP2 on the lysosome membrane. Ref.12 Ref.14. Tissue specificity: Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Isoform 2 also expressed in the sarcolemma of skeletal muscle. Levels of this cellular isoform decreased in prostate cancer. Ref.12 Ref.19. Post-translational modification: N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Ref.9 Ref.21 Ref.22Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection. Ref.13 Ref.15. Miscellaneous: Used as a diagnostic tool for staging metastatic prostatic cancer. Sequence similarities: Belongs to the histidine acid phosphatase family.

size :

1 mg

price :

140 USD

more info or order :

MyBioSource product webpage