protein

GAPDH protein

MBS537767

0.1 mg

460 USD

Native Protein

GAPDH protein

GAPDH

OTTHUMP00000174431 protein; KNC-NDS6 protein; Glyceraldehyde-3-phosphate dehydrogenase protein; OCAS protein; Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate protein; EC 1.2.1.12 protein; GAPD protein; cb609 protein; wu:fb33a10 protein; GAPDH protein; G3PDH protein; Aging-associated gene 9 protein protein; OTTHUMP00000174432 protein; MGC105239 protein; p38 component protein; MGC88685 protein; MGC103191 protein; MGC103190 protein; 38-KD component protein; Glyceraldehyde 3 phosphate dehydrogenase protein; OCT1 coactivator in S phase protein; MGC102544 protein; MGC127711 protein; BARS-38 protein; MGC102546 protein; G3PD protein

glyceraldehyde-3-phosphate dehydrogenase isoform 1; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

GAPDH

GAPDH; GAPDH; G3PD; GAPD; HEL-S-162eP; GAPD; GAPDH

G3P_HUMAN

Human

335

> 98% pure

GAPDH protein is supplied in 80% ammonium sulphate with traces of Hepes, EDTA and DTT.

Store at 4 degree C.

User optimized

Source Note: Human heart tissue . Protein Type: Native. Biological Significance: GAPDH also known as Glyceraldehyde 3-phosphate dehydrogenase is an enzyme responsible for catalyzing a vital step in the glycolysis pathway. The main purpose of GAPDH is to break down glucose for energy and as a source of carbon. GAPDH also plays an important role in several non-metabolic processes, including activation of transcription, apoptosis initiation, and cell membrane transport

Biohazard Information: Donor samples were tested and found to be negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis. Nonetheless caution should be used when handling this material as there is a margin of error in all tests.

Proteases, Inhibitors, Native Protein

Purified native Human GAPDH protein

7669492

NP_002037.2

NM_002046.4

P04406

31,548 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Antibiotics Pathway (1144997); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342)

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma. Protein type: Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.12. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: microtubule cytoskeleton; nuclear membrane; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipid particle; ribonucleoprotein complex; nucleus; cytosol; vesicle. Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding. Biological Process: neuron apoptosis; glycolysis; protein stabilization; negative regulation of translation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; microtubule cytoskeleton organization and biogenesis; gluconeogenesis

0.1 mg

460 USD