protein

5-Lipoxygenase protein

MBS537729

500 Units

570 USD

Recombinant Protein

5-Lipoxygenase protein

5-Lipoxygenase

5-Lipoxygenase Antigen; 5 Lipoxygenase Recombinant Protein

5-lipoxygenase, partial; Arachidonate 5-lipoxygenase; arachidonate 5-lipoxygenase; arachidonate 5-lipoxygenase

ALOX5; ALOX5; 5-LO; 5LPG; LOG5; 5-LOX; LOG5; 5-LO; 5-lipoxygenase

LOX5_HUMAN

Human

50

50mM Tris containing 5mM EDTA, pH 8.0.

1619.3 units/ml. 9.94mg/ml. Specific Activity: 162.9 Units per mg Protein.

Aliquot and store at -70 degree C or lower. Avoid repeated freeze/thaw cycles

User optimized

Protein Type: Recombinant. Biological Significance: 5-lipoxygenase (5-LO) catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to. Bioactivity: 20 units/mg protein - One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25 degree C in 50 mM Tris-HCl, 100 uM arachidonate, 2 mM CaCl2, 1 mM ATP, 250 uM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.

Expression System: Baculovirus. Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Proteases, Inhibitors, & Enzymes

Purified recombinant Human 5-Lipoxygenase protein

187167

AAA63212.1

P09917

78 kDa

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); IL-5 Signaling Pathway (198770); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Ovarian Steroidogenesis Pathway (790011); Ovarian Steroidogenesis Pathway (795173)

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer. Protein type: EC 1.13.11.34; Oxidoreductase; Nuclear envelope; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol. Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity. Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process. Disease: Asthma, Susceptibility To

500 Units

570 USD