catalog number :
MBS537040
products type :
Native Protein
products full name :
NSE protein
products short name :
[NSE]
products name syn :
[Neural enolase protein; Enolase 2 gamma neuronal protein; Neuron Specific Enolase protein; Neuron specific enolase protein; Enolase 2 protein; gamma-enolase protein; Enolase2 protein; Neuron specific gamma enolase protein; gamma enolase protein]
other names :
[neuron-specific enolase; Gamma-enolase; gamma-enolase; enolase 2, gamma, neuronal; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE]
products gene name :
[NSE]
other gene names :
[Eno2; Eno2; NSE; RNEN3; Eno-2; NSE]
uniprot entry name :
ENOG_RAT
purity :
> 95% by SDS-PAGE. NSE protein was purified using Ion-exchange chromatography and gel-filtration.
form :
Frozen liquid in 100mM Tris-HCl, with 5 mM Mg 2 SO 4 , and 250mM KCl, pH 5.1 adjusted with sodium phosphate.
concentration :
0.81mg/ml
storage stability :
Aliquot and store at -20 degree C. Avoid repeated Freeze/Thaw cycles. Shipping: Ships with Dry Ice.
tested application :
User optimized
image1 heading :
SDS-PAGE
other info1 :
Source: Post mortem human brain . Protein Type: Native. Biological Significance: Neuron-specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. Detection of NSE with antibodies can be used to identify neuronal cells and cells with neuroendocrine differentiation.
other info2 :
Biohazard Information: Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies and syphilis. Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
products categories :
Neuroscience; Native Protein
products description :
Purified native Human NSE protein
ncbi acc num :
AAB72088.1
ncbi mol weight :
47,141 Da
ncbi pathways :
Biosynthesis Of Amino Acids Pathway (791250); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Antibiotics Pathway (1146234); Carbon Metabolism Pathway (816129); Carbon Metabolism Pathway (817567); Gluconeogenesis Pathway (1178045); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (434661); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (1178043); Glycolysis Pathway (1178044)
ncbi summary :
catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate; may play a role in glycolysis [RGD, Feb 2006]
uniprot summary :
ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. Protein type: Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11. Cellular Component: extracellular space; neuron projection; photoreceptor inner segment; phosphopyruvate hydratase complex; cell soma; cytoplasm; perikaryon; intracellular; myelin sheath. Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity. Biological Process: response to drug; glycolysis; response to organic cyclic substance; response to estradiol stimulus; gluconeogenesis